164,577 results

1. MSP955

   Plasmid

   #98784

   Purpose

   Accessory Plasmid for PACE

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   proB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCyCBE

   Plasmid

   #226505

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

   Depositor

   Weizhong Chen

   Article

   Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

   Inserts

   APOBEC1-Cas9D10A
   sgRNA
   sacB gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TRPV1exCellHalo

   Plasmid

   #220307

   Purpose

   Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

   Depositor

   Eric Senning

   Article

   Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

   Insert

   rTRPV1exCellHalo (Trpv1 Rat)
   

   Use

   Tags

   circularly permutated Halotag (Deo et al., Nat Ch…

   Expression

   Mammalian

   Mutation

   linker included to insert cpHaloTag (

   Promoter

   CMV promoter

   Available Since

   July 30, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRSFDUET-1_VNp-anti-GFP mAb

   Plasmid

   #214955

   Purpose

   Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-GFP mAb heavy chain
   VNp-anti-GFP mAb light chain
   

   Use

   Tags

   VNp6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP14467 - pAAV-AiE2182m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4467)

   Plasmid

   #224043

   Purpose

   AiE2182m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT neurons (strong)

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF3054

   Plasmid

   #144530

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SALL4 (SALL4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. nLbCas12a

   Plasmid

   #213745

   Purpose

   For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   nLbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dead-Laconic/pcDNA3.1(-)

   Plasmid

   #118627

   Purpose

   To express in mammalian cells a lactate FRET sensor negative control.

   Depositor

   Luis Felipe Barros

   Article

   Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

   Insert

   Dead-Laconic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Change Histidine 151 to Aspartic Acid of Escheric…

   Promoter

   CMV Promoter

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-dLight1.3b

   Plasmid

   #111056

   Purpose

   Expresses dLight1.3b in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.3b
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-GRAB_SST1.0

    Plasmid

    #208664

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-25xAARE-minP-EGFP

    Plasmid

    #159666

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xAARE-minP-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6MFV SLC25A4

    Plasmid

    #194133

    Purpose

    Expresses ANT1 (SLC25A4) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC25A4 (SLC25A4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINTO-N3::hTET3

    Plasmid

    #136364

    Purpose

    Expresses epitope-tagged human TET3 WT in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Use

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-AIF

    Plasmid

    #16182

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Apoptosis-inducing factor (AIFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid

    #225684

    Purpose

    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

    Depositor

    Ryan Mehl

    Inserts

    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    

    Use

    Methanomethylophilus alvus

    Tags

    Expression

    Mutation

    CTG125CTT, N166A, V168S, A223C, W239R

    Promoter

    GlnS and Lpp

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. RP4-8

    Plasmid

    #79813

    Purpose

    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Gentamicin resistance gene
    

    Use

    Helper bhr plasmid for mobilizing other plasmids …

    Tags

    Expression

    Mutation

    Promoter

    Own promoter

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pIntegrase_8

    Plasmid

    #60579

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - EGFP sgRNA 2

    Plasmid

    #51761

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCnCre

    Plasmid

    #232907

    Purpose

    Electroporation vector for expression of Cre recombinase in Cuprividus necator

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    Cre recombinase (cre Synthetic)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ptac-cymO

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pReporter_8

    Plasmid

    #60568

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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