We narrowed to 3,108 results for: N-EGFP

1. GB4080

   Plasmid

   #215249

   Purpose

   Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-EGFP-p19-LUZ-H3H-HISPS-CPH
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCL1032

   Plasmid

   #217962

   Purpose

   Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.

   Depositor

   Andrew Bowman

   Article

   Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

   Insert

   NASP (NASP Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

   Plasmid

   #174424

   Purpose

   Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(Thrombin)EGFP(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCellFree_KA1_ZIKVC

   Plasmid

   #145339

   Purpose

   Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Capsid
   

   Use

   Tags

   8xHis and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available Since

   April 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrichoGate-8

   Plasmid

   #160708

   Purpose

   For N-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   Trichoderma optimised Synthetic eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SMT503

   Plasmid

   #226840

   Purpose

   Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp

   Depositor

   Polly Fordyce

   Article

   Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

   Insert

   Lipase A
   

   Use

   Synthetic Biology

   Tags

   SNAP and eGFP

   Expression

   Bacterial

   Mutation

   R33Q, D34N, K35D, K112D, M134D, Y139C, I157M

   Promoter

   T7

   Available Since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB4807

   Plasmid

   #215273

   Purpose

   Module for the expressino of the N. nambi HISPS gene under the PNOS promoter and the constitutive expression of EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   PNOS:HISPS-SF-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GB4808

   Plasmid

   #215274

   Purpose

   Module for the expressino of the N. nambi HISPS gene under the P35S promoter and the constitutive expression of EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   P35S:HISPS-SF-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GB4468

   Plasmid

   #215257

   Purpose

   Module for the constitutive expression of the N. nambi LUZ, H3H genes, EGFP and p19 fused to a SF in omega2.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCellFree_G01

    Plasmid

    #67135

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSiMPls_N + pSiMPls_C

    Plasmid

    #167203

    Purpose

    SiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycin

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al ACS Omega. 2021 May 21;6(22):14148-14153

    Inserts

    EGFP
    SmR 1-75 + gp41-1 N-intein
    mRuby3
    gp41-1 C-intein + SmR 76-263
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR, CAT, pBAD, and pTrc

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SpdCas9-VP64

    Plasmid

    #115794

    Purpose

    Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    VP64
    

    Use

    AAV

    Tags

    3XFLAG and dCas9-VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IB-pSu9-HaloTag7-mGFP

    Plasmid

    #184905

    Purpose

    Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    Presequence of Fo-ATPase subunit 9
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid

    #184902

    Purpose

    Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7, monomeric EGFP, and monomeric RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRX-IB-HaloTag7-mGFP-KDEL

    Plasmid

    #184904

    Purpose

    Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    KDEL
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMS2-GFP

    Plasmid

    #27121

    Depositor

    Robert Singer

    Article

    Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

    Insert

    MS2
    

    Use

    Tags

    EGFP, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-puro GFP-DFCP1

    Plasmid

    #38269

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RV-Cag-Dio-GFP

    Plasmid

    #87662

    Purpose

    retroviral vector encoding cre dependent expression of GFP

    Depositor

    Oscar Marin

    Article

    Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FHUG+W (B2)

    Plasmid

    #74011

    Purpose

    lentiviral expression of EGFP and shRNA (from H1 promoter, empty)

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJA317

    Plasmid

    #74488

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d1ODC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge