We narrowed to 11,985 results for: pcdna

1. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

   Plasmid

   #169333

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CHRNA7-mGFP

   Plasmid

   #62629

   Purpose

   mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   hAlpha7 (CHRNA7 Human)
   

   Use

   Tags

   mEGFP inserted in M3-M4 loop

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GGamma5-GFP2

   Plasmid

   #166777

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma5-GFP2 (GNG5 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GGamma3-GFP2

   Plasmid

   #166775

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma3-GFP2 (GNG3 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-AMBRA1-3xFLAG

   Plasmid

   #172605

   Purpose

   Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA2-CUL4A

   Plasmid

   #19907

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   Cullin 4A (CUL4A Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   K644R

   Promoter

   CMV

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1his p66shc (corrected)

   Plasmid

   #32574

   Depositor

   Toren Finkel

   Article

   Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

   Insert

   p66shc (SHC1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-myc3-L11

   Plasmid

   #20936

   Depositor

   Yue Xiong

   Article

   Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

   Insert

   Ribosomal protein L11 (RPL11 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA4.1-Myc-RNF8

   Plasmid

   #221676

   Purpose

   Mammalian expression of Myc-tagged human RNF8

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   RNF8 (RNF8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-FLAG-RIPK1

    Plasmid

    #78842

    Purpose

    To overexpress RIPK1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-GGamma7-GFP2

    Plasmid

    #166778

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma7-GFP2 (GNG7 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-SC35-cMyc

    Plasmid

    #44721

    Depositor

    Kathleen Scotto

    Article

    Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

    Insert

    SC35 (SRSF2 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1.Gαi2-LgB91

    Plasmid

    #134358

    Purpose

    Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi2-LgB91 (GNAI2 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6 asyn E46K

    Plasmid

    #105730

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E46K

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1.Gα12-LgB115

    Plasmid

    #134363

    Purpose

    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα12-LgB115 (GNA12 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO_HaloTag7_LamB1

    Plasmid

    #175528

    Purpose

    Expression of HaloTag7 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-AKAR4-Kras

    Plasmid

    #61621

    Purpose

    Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4-Kras
    

    Use

    Tags

    C-terminal targeting sequence from Kras, Cerulean…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-STAT3-S727D

    Plasmid

    #73364

    Purpose

    Expression of STAT3-S727D

    Depositor

    Jie Chen

    Article

    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

    Insert

    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    

    Use

    Tags

    FLAG tagged

    Expression

    Mammalian

    Mutation

    E16K S727D

    Promoter

    CMV

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-ALK6 Q203D

    Plasmid

    #80883

    Purpose

    mammalian expression of ALK6 Q203D

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK6 (Bmpr1b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q203D (constitutively active)

    Promoter

    CMV

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA4-STRAD alpha

    Plasmid

    #14889

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    STRAD alpha (STRADA Human)
    

    Use

    Tags

    6xHis/Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only