We narrowed to 2,115 results for: PORA

1. Mm-PylRS-AF/Pyl-tRNACUA

   Plasmid

   #122650

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   PylRS-AF, Pyl-tRNACUA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SepRS(2)/pSertRNA(B4)/EF-Sep

   Plasmid

   #173897

   Purpose

   The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

   Depositor

   Jason W Chin

   Article

   Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

   Inserts

   SepRS(2)
   pSertRNA
   EF-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac1.tR4-MbPyl

   Plasmid

   #50832

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Pyl tRNA cassette
   MbPyl
   two copy Pyl tRNA cassette
   

   Use

   Baculoviral

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CMV and U6 and H1

   Available since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEvol-MjaYRS

   Plasmid

   #153557

   Purpose

   Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

   Depositor

   Huiwang Ai

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

   Inserts

   MJaYRS (first copy)
   MJaYRS (second copy)
   amber suppression tRNA under proK promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   glnS and pBAD

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEVOL_PylRS(WT)-tRNA

   Plasmid

   #223512

   Purpose

   PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD, rrnB, and glnS and proK

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule2-para-aminoPhe

   Plasmid

   #85503

   Purpose

   Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

   Insert

   p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32T, E107T, D158P, I159L, L162A

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pyronic/pcDNA3.1(-)

   Plasmid

   #51308

   Purpose

   Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

   Insert

   Pyronic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF-G1TFAKRS

    Plasmid

    #177311

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFN18A-HaloTag-Biotin

    Plasmid

    #206039

    Purpose

    HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Insert

    Talin R3-IVVI (Tln1 Mouse)
    

    Use

    Tags

    10 x His-AviTag and Halo-Tag

    Expression

    Bacterial

    Mutation

    Threonine 809 to Isoleucine, Threonine 833 to Val…

    Promoter

    T7

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDule-para-aminoPhe

    Plasmid

    #85502

    Purpose

    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

    Insert

    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32T, E107T, D158P, I159L, L162A

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-G1(4CNW)RS

    Plasmid

    #222871

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEVOL-AcKRS-CloDF

    Plasmid

    #127415

    Purpose

    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR-SF61

    Plasmid

    #163916

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277

    Insert

    SF5Phe tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-cTNT-Cre-miR122 TS

    Plasmid

    #209784

    Purpose

    Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    miR122 ts
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS_375

    Plasmid

    #182132

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Use

    Tags

    Expression

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEDF-PhdRS

    Plasmid

    #127445

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A mutations on PylRS

    Promoter

    araBAD

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule2-IBBN (G2)

    Plasmid

    #85501

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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