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We narrowed to 19,203 results for: IRE

Showing: 4901 - 4950 of 19203 results
  1. pDEST-pSP172BSSPE-Swa1::RfA-HA

    Plasmid
    #186405
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pR6Kg-Alpha2

    Plasmid
    #165863
    Purpose
    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pMM0649

    Plasmid
    #129010
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pMM0655

    Plasmid
    #129016
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter
    Depositor
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pMM0654

    Plasmid
    #129015
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter
    Depositor
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pMM0653

    Plasmid
    #129014
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pMM0650

    Plasmid
    #129011
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter
    Depositor
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pMM0648

    Plasmid
    #129009
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects
    Depositor
    Insert
    5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pMM0651

    Plasmid
    #129012
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter
    Depositor
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pGS1112

    Plasmid
    #63784
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker
    Insert
    eSapphire-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    Sapphire-Strep-tag II
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. pCAGGS_3E5E_eGFP

    Plasmid
    #103054
    Purpose
    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Insert
    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    Tags
    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. pHDR-USR-SpCas9

    Plasmid
    #208829
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid
    #123362
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.
    Depositor
    Insert
    Firefly luciferase
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. pSLGCV

    Plasmid
    #49862
    Purpose
    Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).
    Insert
    firefly luciferase luc+ fused to GFP (S65C)
    Tags
    GFP (S65C)
    Expression
    Worm
    Promoter
    sur-5
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
  16. 908H

    Plasmid
    #160240
    Purpose
    TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Inserts
    Tet Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. 908E

    Plasmid
    #160238
    Purpose
    TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Inserts
    Ttg Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. 908G

    Plasmid
    #160239
    Purpose
    PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Inserts
    Pip Operator (3x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. 908J

    Plasmid
    #160242
    Purpose
    CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Inserts
    Cym Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pcDNA3.1-VcnTS-E1015A

    Plasmid
    #213415
    Purpose
    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
    Depositor
    Insert
    VcnTS-E1015A (VCL Synthetic, Chicken)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pcDNA3.1-VcnTS-E1021A

    Plasmid
    #213416
    Purpose
    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
    Depositor
    Insert
    VcnTS-E1021A (VCL Synthetic, Chicken)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1021 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. pcDNA3.1-VcnCS-E1015A

    Plasmid
    #213412
    Purpose
    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
    Depositor
    Insert
    VcnCS-E1015A (VCL Synthetic, Chicken)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  23. pcDNA3.1-VcnCS-E1021A

    Plasmid
    #213413
    Purpose
    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
    Depositor
    Insert
    VcnCS-E1021A (VCL Synthetic, Chicken)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1021 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  24. pENT-L1-Kozak-Ark-L2

    Plasmid
    #186362
    Purpose
    Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
    Depositor
    Insert
    Ciona robusta Aurora kinase A
    Use
    Expression of a fluorescent centrosome marker
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 4901 - 4950 of 19203 results