We narrowed to 5,415 results for: PEP

1. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

   Plasmid

   #230059

   Purpose

   Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 single chain variable fragment (scFv)
   

   Use

   Tags

   6X HIS, HRV 3C PreScission protease, HaloTag, and…

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-17X-PTDH

   Plasmid

   #166786

   Purpose

   Phosphite dehydrogenase for NAD(P)H regeneration

   Depositor

   Wilfred van der Donk

   Article

   Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

   Insert

   Phosphite dehydroenase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A196R, T201S, A328T, E352N, and C356D- please see…

   Promoter

   T7

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPROEX Hsp104 (1-575)

   Plasmid

   #1318

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 (1-575) (HSP104 Budding Yeast)
   

   Use

   Tags

   6X His + TEV cleavage

   Expression

   Bacterial

   Mutation

   aa1-575 of Hsp104

   Promoter

   Available since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b-CoilR-Cys

   Plasmid

   #181989

   Purpose

   Used to label proteins with the CoilE genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

   Insert

   CoilR-Cys
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAP24

   Plasmid

   #70190

   Purpose

   Modular vector for secreted luciferase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   sLucopt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP.R265

   Plasmid

   #138167

   Purpose

   Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(VDA).M86.(SDL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMT-PT2A-iRFP670-Tdtomato-GFP

   Plasmid

   #111817

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   Tdtomato
   GFP
   

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDS257

   Plasmid

   #34971

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPT2

   Plasmid

   #209974

   Purpose

   Contains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Terminator (T_pepN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBGSA HA-FPR1

    Plasmid

    #62603

    Purpose

    Constitutive expression of HA-FPR1 in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

    Insert

    FPR1 (FPR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pHH0103_NR1D1_Hormone-recep

    Plasmid

    #109960

    Purpose

    Protein expression and purification of NR1D1_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1D1_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEm-CoilE-Actin

    Plasmid

    #181993

    Purpose

    CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    mEm-CoilE-Actin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXylA-agrCA-I

    Plasmid

    #53437

    Purpose

    xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrCA locus
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    "MVQTS" sequence added to the N-terminu…

    Promoter

    PxylA

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST14-SpyCatcher EQ

    Plasmid

    #35045

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher EQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

15. MUNC18-2

    Plasmid

    #186687

    Purpose

    Expresses MUNC18-2 in insect cells

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    MUNC18-2 (Stxbp2 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-HA-GlpG

    Plasmid

    #155135

    Purpose

    Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFP.R272

    Plasmid

    #138221

    Purpose

    Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDS277

    Plasmid

    #34973

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V529N

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_NR6A1_Hormone-recep

    Plasmid

    #109945

    Purpose

    Protein expression and purification of NR6A1_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR6A1_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFP.R364

    Plasmid

    #138234

    Purpose

    Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge