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Plasmid#109955PurposeProtein expression and purification of PPARD_Hormone-recepDepositorInsertPPARD_Hormone-recep
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
UseTagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSO7
Plasmid#106365PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.DepositorInsertttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (773-908)
Plasmid#1316DepositorInsertHsp104 (773-908) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationdeletion of aa1-773PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (361-548)
Plasmid#1317DepositorInsertHsp104 (361-548) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa361-548 of Hsp104PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
mNG-mCh
Plasmid#124215PurposeCytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mChDepositorInsertmNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-FW/AA
Plasmid#12293DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y, F774A and W775A; signal peptide (aa1…PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJLI-SUP35 (delta)2-5
Plasmid#1138DepositorInsertsup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationlast four repeats of sup35 N domain deleted, but …PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-GFP-iRFP670
Plasmid#111814PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
GFP
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-DIO-Vgf-T2A-mCherry-CW3SL
Plasmid#223661PurposeExpresses VGF and mCherry in a Cre-dependent mannerDepositorInsertVgf-T2A-mCherry
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-flx-fDIO-HA-Rpl10a-T2A-myc-hM3D(Gq)
Plasmid#223654PurposeExpresses vTRAP and hM3D(Gq) in a FlpON-CreOFF dependent mannerDepositorInsertvTRAP-T2A-hM3D(Gq)
UseAAVTagsHAExpressionMutationPromoterhSynAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
PC2328
Plasmid#232089PurposeMammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.DepositorInsertduAb with cloning Esp3I restriction site
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DD-T6B-WT-YFP
Plasmid#235143PurposeExpresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoterDepositorInsertDHFR-fused T6B peptide
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-L7-6-DD-T6B-WT-YFP
Plasmid#235145PurposeExpresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoterDepositorInsertDHFR-fused T6B peptide
UseAAVTagsExpressionMutationPromoterL7-6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-L7-6-FLAG-HA-T6B-WT-YFP
Plasmid#235141PurposeExpresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoterDepositorInsertT6B peptide
UseAAVTagsFLAG/HAExpressionMutationPromoterL7-6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLAG-HA-T6B-WT-YFP
Plasmid#235139PurposeExpresses the T6B peptide fused with YFP under the human synapsin promoterDepositorInsertT6B peptide
UseAAVTagsFLAG/HAExpressionMutationPromoterhSynAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-ppiB
Plasmid#202482PurposeExpresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletionDepositorInsertpeptidyl-prolyl cis-trans isomerase (ppiB)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-ubi-Y-pyrG
Plasmid#184497PurposeAMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-coSnaA-TEV-His_His-TEV-SnaC
Plasmid#225059Purposerecombinant expression of SnaA in E. coliDepositorInsertSnaA
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only