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Showing: 1 - 20 of 1183 results
  1. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Mutation
    Promoter
    SP6
    Availability
    Academic Institutions and Nonprofits only
  2. pMB70

    Plasmid
    #47943
    Purpose
    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences
    Depositor
    Insert
    guide RNA
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pMB62

    Plasmid
    #47944
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter
    Depositor
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, egl-13 NLS, mEGFP, and SV40 NLS
    Expression
    Worm
    Mutation
    Promoter
    eef-1A.1 (eft-3)
    Availability
    Academic Institutions and Nonprofits only
  4. pMB63

    Plasmid
    #47945
    Purpose
    Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter
    Depositor
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, egl-13 NLS, and SV40 NLS
    Expression
    Worm
    Mutation
    Promoter
    eef-1A.1 (eft-3)
    Availability
    Academic Institutions and Nonprofits only
  5. pMB66

    Plasmid
    #47946
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter
    Depositor
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, egl-13 NLS, SV40 NLS, and mEGFP
    Expression
    Worm
    Mutation
    Promoter
    hsp-16.48
    Availability
    Academic Institutions and Nonprofits only
  6. pMB67

    Plasmid
    #47947
    Purpose
    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
    Depositor
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, egl-13 NLS, and SV40 NLS
    Expression
    Worm
    Mutation
    Promoter
    hsp-16.48
    Availability
    Academic Institutions and Nonprofits only
  7. pSLGCV

    Plasmid
    #49862
    Purpose
    Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).
    Insert
    firefly luciferase luc+ fused to GFP (S65C)
    Use
    Tags
    GFP (S65C)
    Expression
    Worm
    Mutation
    Promoter
    sur-5
    Availability
    Academic Institutions and Nonprofits only
  8. pCFJ1655

    Plasmid
    #51481
    Purpose
    miniMos transposable element with HygroR, R4-R3 Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pCFJ1662

    Plasmid
    #51482
    Purpose
    miniMos transposable element with HygroR, MCS cloning
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pCFJ1656

    Plasmid
    #51483
    Purpose
    miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pCFJ1663

    Plasmid
    #51484
    Purpose
    miniMos transposable element with HygroR and peel-1, MCS cloning
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pCFJ1666

    Plasmid
    #51485
    Purpose
    miniMos transposable element with PuroR, MCS cloning
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 1183 results