Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 783 results for: post

Showing: 31 - 60 of 783 results

  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (96 h post-transfection): Add valproic... Feed. Tuesday (120 h post-transfection): Add 4% BCD Feed. Wednesday (144 h post-transfection): Add 4%...168 h post-transfection): Harvest antibody. Section 4: Harvest antibody At 168 hours (1 week) post-transfection...... Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology Reference General Transfection...valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% BCD Feed...a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM ...

  2. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...protocol for coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization...

  3. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base editors — cytosine base editors...

  4. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  5. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  6. Retrovirus Plasmids

    Type
    Collection
    ...GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that increases...

  7. Antibody Production

    Type
    Collection
    ... Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

  8. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  9. Microbiology Resources

    Type
    Collection
    ...CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making this possible...

  10. Worm Expression Resources

    Type
    Collection
    ...elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing...

  11. Plant Plasmids and Resources

    Type
    Collection
    ...Promoter PI Return to top Other Resources Addgene Blog Posts RUBY-Red Siliques CRISPR-mediated Plant Base Editors...

  12. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present a video reprint from...Tables and Figures, please access the manuscript PDF posted at the top of the page. CRISPR Design Design sgRNAs...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine...

  14. Zebrafish Plasmid Collection

    Type
    Collection
    ...proteins. Return to top Other Resources Addgene Blog Posts Advancing Biology with Zebrafish: Genetic Tools ...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Biosensors Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal Junctions Secretory...pathway require chaperones to help with folding or post-translational modification to function properly....

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  18. Fluorescence Titering Assay

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This... of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed for mCherry expression...

  19. AAV ddPCR Titration

    Type
    Protocol
    ...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...tips on AAV titering using ddPCR, read this blog post on ddPCR for AAV quantification. This protocol was... tips on AAV titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

  20. General Transfection

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace with fresh ...

  21. Lentivirus Production

    Type
    Protocol
    ...(pm): Transfect packaging cells Day 2 (am): 18 h post-transfection. Remove media, replace with fresh media...cells. Virus can be harvested at 48, 72, and 96 h post transfection in individual harvests or a combined...

  22. Plan Your Experiment

    Type
    Collection
    ...each of these techniques can be found in our blog post CRISPR 101: Validating your Genome Edit . Publications...

  24. Video Library

    Type
    Protocol
    ...for working with tissue culture in the lab. Blog post: 10 Basic tips for mammalian cell culture Aseptic...

  25. AAV Production in HEK293 Cells

    Type
    Protocol
    ...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...120 h. Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

  26. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful tool for genetic screening experiments...

  27. Zhang Lab CRISPR Page

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo...

  28. Immunocytochemistry

    Type
    Protocol
    ...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western Blot Protocol Recombinant...

  29. Plasmids for Stem Cell Research

    Type
    Collection
    ...difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an...

  30. Colony Formation Titering Assay

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...
Showing: 31 - 60 of 783 results