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We narrowed to 370 results for: Cre expression vector

Showing: 321 - 360 of 370 results

  1. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...cell type-specific expression. The Allen Institute for Brain Sciences has screened thousands of enhancers...Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m...224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_IT Isocortex 224038 ...220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 ...220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 ...

  2. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  5. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX...pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B...101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag ...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Synthases (AcpS and Sfp, respectively) may be expressed and purified as described previously in Stachelhaus...-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies...

  6. Promega Plasmid Collection

    Type
    Collection
    ...luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such...luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for detecting ... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...enables highly sensitive detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular...want minimal disruption of endogenous protein expression and interactions, such as exploring viral biology...HaloTag technology is optimized for various protein expression systems including E. coli , mammalian cells, ...interest is fused to either LgBiT or SmBiT and expressed in live cells. When the two proteins interact,...

  7. Biosensor AAV Preps

    Type
    Collection
    ...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent... none Cre dependent 1, 9 Kim , GENIE 100839 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 CAG GCaMP6m none Cre dependent...

  8. CRISPR Plasmids - Plants

    Type
    Collection
    ...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in plants. CRISPR...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  9. Open Enzyme Collection

    Type
    Collection
    ...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...Other Enzymes You may also like… Bacterial Expression Vectors Synthetic Biology Plasmids BIOFAB Plasmid...their own enzymes by cloning them into an expression vector of their choice and transforming into E. coli... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...and codon-optimized the Open Enzymes parts for expression in Escherichia coli bacteria. Researchers can...purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all plasmids in ... are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new window) — all Open...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, and Drosophila...another round. Figure 1: Overview of prime editing. Created with BioRender.com. The original prime editing ...

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    .... PubMed Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available...available on our FRET Resource page and can be used to create fusions with a gene of interest. These plasmids...biosensors too, as another laboratory may have already created the sensor that you need. FRET biosensors designed... endogenous state that you want to measure (overexpressing a gene or protein after transfection). Future...multiple FRET biosensors simultaneously would further increase the amount of correlated information available...

  12. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... plasmids for activating or upregulating gene expression in various model organisms. CRISPR...transcriptional activation. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  13. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...transcriptional inhibition. Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  14. CRISPR Plasmids and Resources

    Type
    Collection
    ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...

  15. Chemogenetics Plasmids

    Type
    Collection
    ...Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics Chemogenetics...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags Eligible for Viral Packaging...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...

  16. Distribution to Industry

    Type
    Collection
    ...while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse ...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's Disease.... How to Order Creating an Account: Before placing your order, you will need to create an account and ...cloning and assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit ... our website. Placing an Order: After you have created an account, you can place orders through our website...

  17. Cancer Research Plasmids and Resources

    Type
    Collection
    ...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson , and Root Labs...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...signaling studies, including mutant, knockdown, and overexpression constructs. Each collection page listed below... Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer...

  18. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids...Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...

  19. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

  20. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA expression vectors available from Addgene....Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...activity. The variety in sequence decreases the gRNA activity and increases off-target effect. Which species...requirements, experimental considerations, and more, created and maintained by the Doudna Lab . gRNA Plasmids...

  21. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and mechanisms.... PMID: 36893201 (Link opens in a new window) Increasing access for biochemistry research in undergraduate...

  22. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...purify genomic loci. Plasmids are available for expression in mammalian systems, bacteria, and yeast. Mammalian...

  23. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Further advancements have been made by optimizing expression of the fusions, modifying the linker region between... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, yeast...double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive...converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand..., which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases...deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA...

  24. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...

  25. CRISPR Guide

    Type
    Collection
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... gRNAs will be expressed in the same cell. This is called multiplexing , and increases the likelihood ...lentivirus or use a Cas9-expressing stable cell line. After applying your screening conditions to your transduced...also been used for genome-wide screens to activate or repress gene expression in mice and human cells. The...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...

  26. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...

  27. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... by expressing and purifying the phosphosite library as GST-fusion peptides (“Mode #1” expression). The...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...characteristics, and an overall improved ease of use when expressing recombinant phosphoproteins. These attributes... origins. Therefore, when choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030...2023) and find general tips on phosphoprotein expression in rEcoli XpS in the Methods for iSPI, rEcoli...phosphorylation-dependent protein-peptide interactions (“Mode #2” expression, Barber et al., 2018). The phosphosite library...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...

  28. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  29. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype...Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service , Addgene is producing...of our standard viral production , these viral vectors undergo quality control, including AAV titration... in vitro as well as in vivo delivery where the vector is diluted by the physiologic body fluids, such...

  30. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  31. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...

  32. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...to introduce a DSB. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...CRISPR plasmids that create double strand breaks in DNA. CRISPR... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  33. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria. Mammalian ...Figure 1: Overview of the basic CAST mechanism. Created with BioRender.com. Browse, sort, or search the...

  34. Genetic Code Expansion

    Type
    Collection
    ...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic...

  35. Fluorescent Proteins: FRET

    Type
    Collection
    ...3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 0.51 530... 128,000 0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET-10 SYFP2...fluorophores through linkers, fluorescent protein expression level and maturation time, and other environmental...imaging microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor...

  36. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled .... Human SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human...pooled libraries are useful tools for genetic screening experiments. These libraries target the solute carrier...

  37. Modular Cloning Guide

    Type
    Guide
    ...Cloning System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing several...Yeast Expression, Insect Expression Chen Davidovich 41 plasmids to construct multigene expression vectors...4G-Cloning Plasmid Kit Bacterial Expression, Insect Expression, Mammalian Expression Stephan Gruber Platform for...assembly of expression vectors for multi-subunit protein complexes in various hosts. Expression cassettes...Toolkit Mammalian Expression Yizhi Cai 95 plasmids to generate mammalian expression vectors for transient ...Assembly Kit Other, Yeast Expression, Plant Expression, Bacterial expression Christopher Dupont 95 plasmids...protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast Expression Brigitte...

  38. Plan Your Experiment

    Type
    Guide
    ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...AAV vectors offer some advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

  39. Adenovirus Guide

    Type
    Guide
    ...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...-generation rAdV vectors. Created with BioRender.com. Second-generation rAdV vectors Second-generation...third-generation rAdV vectors. Figure 3: Second-generation rAdV vectors. Created with BioRender.com. Third-generation...Third-generation rAdV vectors and helper virus. Created with BioRender.com. Adenoviral Vector Production Cloning...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...

  40. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Viral Vectors AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...Addgene’s Viral Vector Protocols . Figure 3: Overview of rAAV vector production. Created with BioRender.com...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...
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