...showed that improved specificity of Cas14 enables high-fidelity SNP genotyping. The development of  Cas14...

...Scientist. I used to spend my days (and often my nights :D) engineering E. coli to produce biofuels in ... screaming from the lab like the cartoon on the right?!? I would love to tell such a dramatic story, but...This may come as somewhat of surprise to many of my high school friends. In my teenage years I was super ...can involve walking alongside it and giving it a high five. While screaming can be therapeutic and I’ve...

...erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 ...

...is not compatible with other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing...

...transgene expression, contains intron that gives you high expression. Trono 12254 pWPI 2nd EF-1alpha driven...

...Mouse Oxycotin mCherry No Geschwind 66795 pAAV-PTRE-tight-hM3Dq-mCherry hM3D (Gq) Tet-inducible mCherry No...

...vector serves as a backbone to clone the left and right homology arms. More details on how to use this plasmid...

... pathways. CrispyCrunch CrispyCrunch is used for high-throughput design and analysis of CRISPR + HDR experiments...

...TALENs can be used to induce targeted mutations with high efficiency in zebrafish ( Sander et al., Nat Biotechnol...

...Biosensors 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn Tight affinity glutamate sensor Glutamate sensor Looger...

... Lin28A to somatic cells from separate plasmids Highly Efficient Reprogramming to Pluripotency and Directed...

...reagent you will be weighing out, a weighing boat or weighing paper to contain the reagent while on the...that you are weighing by looking for a weight range on the scale. Make sure that the weight of the material...out the material you are weighing onto the weighing boat or paper. If you weighed out too much material,... up. Discard the weighing boat or weighing paper in the trash if the material weighed is not biohazardous... Protocols Weighing Reagents Weighing Reagents Protocol Intro ... to handling the materials. Equipment Weighing boats or weighing paper Balance Scoopula or spatula Container...button. Before weighing out your reagents, determine the amount that you need to weigh. Gather the reagent...

...The route the light follows from the source to your eyes is called the light path . Light travels from ... Protocols Using a Light Microscope Using a Light Microscope Intro ... to encounter: the compound light microscope. As the name suggests, light microscopes take advantage of...compound light microscope with the main components labeled: Figure 1: Diagram of a compound light microscope...from the light source through a condenser that helps concentrate the light onto the sample, and then through...objective! Once on the higher objective, you will likely need to re-adjust lighting and focus. Increase ...basics of how to use a light microscope. Protocols...

...coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter un​LOCK​ing)... under either no excitation light, UV light or LED blue light.  Highlights: This is a one-pot, one-temperature...heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the SHERLOCK...captured by an antibody further along on the strip. Highlights: Of the 83 known COVID-positive samples tested... extraction but that may work only if there is a high enough (105 copies per microliter) viral load in...lateral-flow readout slip that allows rapid detection.  Highlights: Cas13a is not catalytically active in the presence... duplex and will not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not...

...Microbial Opsins Opsins are light-gated ion channels or pumps that absorb light at specific wavelengths. ...respond to different wavelengths of light, like blue or yellow light. These various opsins also initiate...also a blue light activated cation channel. ChR2 is preferred over ChR1 because ChR2 has higher conductance...Channelrhodopsin from Volvox carteri 570 Chronos High-speed, light-sensitive channel from Stigeoclonium helveticum... 566 ArchT Light-activated outward proton pump from Halorubrum strain TP009. Improved light sensitivity...binding domain, allowing light to activate transcription. Phytochromes and light oxygen voltage (LOV) domains...) BLUF domains bPAC Light-activated adenylyl cyclase from Beggiatoa sp. for light-induced cAMP modulation...

...enough to cause a DSB. If high specificity is crucial to your experiment, you might consider using the dual...will have the highest specificity. To increase specificity, you can also consider using a high-fidelity Cas...Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 8(11):2281-308. PMID: 24157548 High-frequency...end joining (NHEJ) pathway The less efficient but high-fidelity homology directed repair (HDR) pathway ...Break (Cut) Enhancing Specificity with Nickases and High Fidelity Enzymes CRISPR specificity is partially...researchers used rational mutagenesis to develop two high fidelity Cas9's : eSpCas9(1.1) and SpCas9-HF1. eSpCas9...interactions with the DNA phosphate backbone. Another high fidelity Cas9, HypaCas9 , was developed in 2017 ...

...the Fab fragments contain highly variable regions, shown in light blue and light orange, that bind to a ...are light-sensitive molecules which emit a specific wavelength of light after excitation by light of a...pair the brightness of the fluorophore with the abundance of the antibody target. If a bright fluorophore...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...-efficient way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies...protein chains: two large heavy chains and two small light chains. These four domains come together to form... to be produced in a single individual, giving a high probability that an antibody will be able to recognize...

...These episomes can concatemerize, producing high molecular weight structures that are maintained extrachromosomally... a hybrid capsid derived from eight serotypes. AAV-DJ displays a higher transduction efficiency in vitro...separation of Rep and Cap shown in the figure on the right facilitates the viral pseudotyping discussed below... of transgenes, as well as the expression of neighboring genes. The short coding sequences of channelrhodopsins...any wild type serotype; in vivo , it displays very high infectivity across a broad range of cell types. ...

...insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters can direct ...determines the type of plasmid and dictates how it might be used in the lab. Below are some common plasmid...particles, that can infect your target cells at a high efficiency. Addgene's expanding viral service offers...Next Generation Sequencing (NGS) comes in. NGS is a high-throughput, multi parallel sequencing platform that... Plasmids 101: 5 factors to help you choose the right cloning method Plasmids 101: Restriction Cloning...

...neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially important for dosing...off-target effects in vivo and the receptors exhibited high levels of constitutive activity, making them less...example, a study in rats using an hM4Di receptor might consider an alternative DREADD ligand such as DCZ...BA, Hufeisen SJ, Meltzer HY, Roth BL (2004). The highly efficacious actions of N-desmethylclozapine at ...