...Baylor College of Medicine Gene Vector Core have kindly deposited a series of lentiviral and adenoviral... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...

...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration; See similar plasmid...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors ...

...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. However...Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short... flank the MCS for improved normalization. John Atkins 167576 mpCHECK2 Firefly/Renilla Insertion of 3'...

...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...

...engineered or identified to increase the spectral and kinetic properties as well as improve the expression and...Cre inhibition", "Boyden 2014", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

...Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support ...

...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...assistance at [email protected] . Don’t See What You’re Looking For? At this time, Addgene has made a limited number...

...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...

...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ... Truncated SARS-CoV-2 Spike gene constructs for making VSV pseudovirus Common human coronavirus (OC43,...

...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Recommended Applications PI Don’t See What You’re Looking For? Please let us know what you would like to ...

...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...biological systems for novel functions. Addgene is working with leading synthetic biologists around the world...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

... Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...protocol videos available that may be helpful for working with this plasmid collection. Single cell passaging...

...Guerin, and Leila Haery from Addgene will also be speaking or leading workshops. Tentative Meeting Agenda...resources for this meeting's publication, we are asking participants to share practical information in ...

...accordance with export laws and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide...Engineering blog post to see how Addgene depositors are making this possible in a range of organisms. Find CRISPR...

... You can search the table for features you are looking for, such as the promoter or cloning method of ... You can search the table for features you are looking for, such as the promoter or fluorescent protein...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

...Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT Thermus thermophilus...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon...