... for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify...3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR KO library 174592 Knockout Human...

...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

...growing collection of general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55...diagnostics and treatments for Alzheimer’s disease and related disorders. The site curates information on useful...

...efficiency of Cas9 cleavage is relatively high and the efficiency of HDR is relatively low, a large portion of...recognizes the NG PAM, has increased activity in vitro relative to other Cas9 endonucleases. Read more about Cas9...SpCas9 for specific applications. For example, the relatively large size of SpCas9 (∼4 kb coding sequence) ...

...real Addgenies! Protocols Video Link Description Related Page Making LB Agar Plates Create plates to culture... Protocol How-To Videos Video Link Description Related Page MTA Guide - Requests Your guide to the MTA...

... A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B...terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids available from ... have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene recommends that you select...

...reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and to minimize well-...following: Standard curve: R 2 (coefficient of correlation) ~ 1.0, E (efficiency of PCR) ~100% (90%-110%...

... across the dilutions. RPP30 copies should be relatively constant across samples. In the RRE example below...concentration of RPP30 positive droplets stays relatively even across samples (green). To increase the ...

...Applications CRISPR-Cas Technology and its Applications Related videos Genome Engineering: Basic Principles Genome...

...Engineering Browse the list of available plasmids related to synthetic biology below. If there are plasmids...

...Browse Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial Strains Search...

...Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental...

... recombinant antibodies targeting neuroscience-related proteins....

...arrangement of the Nuclear Localization Signal (NLS) relative to the hCas9 coding region was optimized for the...

...Addgene's collection of synthetic biology plasmids related to metabolic pathways and components. Examples ...

...ongoing project of the Center for Alzheimer’s and Related Dementias (CARD) at the NIH. Reference Ramos et...

...for expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering...

...Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library construction...

...effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that...

...TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: http://www.thesgc.org/ SGC Toronto...