... PI Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...The collection can be used for screening transcription factors for cellular programming or cancer drug ...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that...that simplifies identification of the transcription factor isoform in pooled screens. This collection ...collection and library can be used for screening transcription factors for applications such as cellular programming...

...Targeting dCas9 to transcription start sites is sufficient to repress transcription by blocking initiation...approaches like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the C-terminus of Cas9...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...uses a CRISPR-Cas9n fused to both a reverse-transcriptase and a serine integrase (Bxb1), along with two...initiation. More robust transcriptional repression (CRISPR interference, or CRISPRi) or activation (CRISPRa...target genes can be achieved by fusing dCas9 to transcriptional repressors or activators and targeting promoter...

... Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry... Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry ...Industry Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description... Description Industry Do you have suggestions for other plasmids that should be added to this list? Fill...

...including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters...collection of luciferase plasmids for assays of transcriptional regulation and bioluminescent reporters. Plasmid... protein half-lives, making them useful as transcriptional reporters. However, they are not secreted, ...control plasmid utilizing Firefly luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf...Gateway cloning to test for the presence of transcriptional regulatory region in specific cell types. Used...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc Firefly Insertion of 5' promoter...Vector for investigating regions controlling transcription Debrya Groskreutz 60323 pGL4.23-GW Firefly Insertion...

...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions...-dU6:3gRNA dU6:3 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and... dU6:1 and dU6:3 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and...-dU6:1gRNA dU6:1 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and...-dU6:2gRNA dU6:2 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and...

...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors...human iPSC generation OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts...Progenitor Cells Lentiviral Human Cooperative Transcription Factor Induction Mediates Hemogenic Reprogramming...Oligodendrocyte Progenitor Cells Lentiviral Mouse Transcription factor-mediated reprogramming of fibroblasts...fibroblasts into functional astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1)...Neurons Lentiviral Human Chemical modulation of transcriptionally enriched signaling pathways to optimize the...

...expression in plants: Maize Transcription Factor ORFeome - John Gray. Rice Transcription Factor ORFeome - John... Addgene Blog: Plant Biology CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins...plant transformation vectors - Daniel Voytas. Transcription Factors The following collections can be used...John Gray. Plant transcription factors for Agrobacterium-mediated transient expression or plant transformation...promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return to top Fluorescent...or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to top Other Resources Addgene...Genomics Resource (Link opens in a new window) - Transcriptomic and metabolomic resources for 14 key medicinal...

...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes (Link opens in...polymerases, RNA polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are... cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis. Additionally...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes DNA Ligases Addgene...165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT...Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) 165556 ...Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT...

... ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson ...new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains...expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro synthesis ...strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences within...destination vectors can be used to create TALE-transcription factors and are used at the final assembly step...for generating TALEN mRNAs through in vitro transcription as an alternative. Based on transient double-selection...

...testing transcription factor binding to reporter plasmid pH3U3-zif268 (omega) . Transcription factors ... answer many interesting questions. Does my transcription factor bind to a particular sequence? Do these...for easy western blotting or staining, and RNA transcript tags to monitor RNA localization. Using these...variety of inducible promoters, their associated transcription factors, and reporter genes. pCS-PesaRlux 47640...Lactose/IPTG inducible expression of EsaR, a transcription factor responsive to 3OC6HSL. EsaR derivatives...that element. When this activation requires a transcription factor to bind to a small molecule, the reporter...derivatives as a fusion to RNA polymerase subunit ω. Transcription factor binding to a putative promoter region...

... Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may be useful for detection assays... industry is continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI For more information on these genes...2020.06.23.166397 (Link opens in a new window) reverse transcriptase, protease, and Bst polymerase constructs now...unpublished) Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa...2020.03.29.013342 (Link opens in a new window) Reverse Transcriptase plasmids from Andrew Ellington's lab. High-surety...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...direct dCas9 to a specific locus, avoiding known transcription factor and other protein binding sites. ID Plasmid...

... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...direct dCas9 to a specific locus, avoiding known transcription factor and other protein binding sites. ID Plasmid...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...direct dCas9 to a specific locus, avoiding known transcription factor and other protein binding sites. ID Plasmid...

...Overview of tetracycline regulated inducible transcriptional activation or repression...tetracycline and not to the TRE, permitting transcription. Tetracycline-dependent promoters are developed... of VP16 (virion protein 16), an essential transcriptional activation domain from HSV (herpes simplex ...and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI ...transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast...Annu Rev Microbiol . 1994;48:345-69. PubMed . Transcriptional activation by tetracyclines in mammalian cells..., tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... cut Goldstein 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung 46170 PU6::klp... plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912 SP6-sgRNA-scaffold...sgRNA-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-...

...efficiently block transcription (in bacteria, ~300-fold repression) by interfering with transcriptional elongation...elongation, RNA polymerase binding, or transcription factor binding. The binding specificity is determined jointly...the nuclease-deficient mutant could silence transcription on the gene expression level without genetically...CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut...can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically inactive...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...

...CRISPR plasmids for transcriptional repression of target genes....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...

...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

... the REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

...reverse transcriptase and integrase. Once in a target cell, the virus uses the reverse transcriptase in tow...envelope and packaging plasmids. ID Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that increases export to...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Mammalian U6x2 yes, nick S. pyogenes Puro Jackson pBluescriptSKII+ U6-sgRNA(F+E) empty 74707 Mammalian hU6 none...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit ...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...

... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

...example, search "Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector ...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...are available for gene knockout, as well as transcriptional activation or repression. Combined with the... fused to epigenetic modifiers can modulate transcriptions and create heritable epigenetic marks. Visualize...CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell . 154(2):442-51. PMID: 23849981...

... CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived growth factor 3, pseudogene CR-3, CRIPTO, CRIPTO...nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription factor...RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel reticuloendotheliosis...beta CBF-A, CBF-B, HAP3, NF-YB NFYC nuclear transcription factor Y, gamma CBF-C, CBFC, DKFZp667G242, FLJ45775..., PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2, ULBP4...CCL15 chemokine ligand 14, chemokine ligand 15 transcription unit - CCL15 chemokine (C-C motif) ligand 15...

...Access to the Paper & Protocol Video Download the manuscript here: Bauer et al, 2014 322.2 KB If you have ...referenced Tables and Figures, please access the manuscript PDF posted at the top of the page. CRISPR Design...leading to nonsense mediated decay of the mRNA transcript ( Figure 1B ). Use the example guides for the...representative results and discussion, download the manuscript here: Bauer et al, 2014 322.2 KB Genome engineering...

...KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in processes ...elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression. Continuing collaborative...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

... are currently in our repository. ID Plasmid Description Promoter Expression System PI 8394 p209 pCMV-... Mammalian Luo 47117 pT3TS-Cre for in vitro transcription of Cre lac Bacterial Ekker 47551 pDD104 (Peft...mCherry-p2A-CreERT2-FRT-kan-FRT For in vitro transcription of Cre or to recombine into BAC SP6 Bacterial...to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico Cre activates...and making transgenic mice. ID Plasmid/Article Description Expression System PI See article Brainbow constructs...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250...42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other...

...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific...transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...

...with the viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...Yan Qin Return to Top Small Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor...Frommer Return to Top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors...Clapham Return to Top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux ...in Living Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication Return...