...Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...collection of site specific recombinases. Viral... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging...in-stock AAV preps that encode site-specific recombinases, which can be used to control gene expression...pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI ...

...cloned, expressed and validated in recombinant form. The recombinant monoclonal antibodies or R-mAbs have...149451 Anti-GluK5/Grik5/KA2 kainate receptor [N279B/27R] GluK5/Grik5/KA2 kainate receptor Rat Mouse IgG2a...Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R] MMP9 matrix metalloproteinase-9 precursor Rat ...IgG2a 220411 LRRK2/Dardarin kinase, C-terminus [N241A/58R] LRRK2/Dardarin kinase, C-terminus Human Mouse ... recombinant monoclonal antibody. Cav1.2 Mouse Mouse IgG2a 225372 SynDIG2 (Mus musculus) recombinant monoclonal..., followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection The following...IgG2a 225364 Kv2.1 pS586 (Rattus norvegicus) recombinant monoclonal antibody. Kv2.1 pS586 Rat Mouse IgG2a...

... Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru 199776...for Cre recombinase expression in striatal direct pathway D1-MSNs Jonathan Ting Viviana Gradinaru 199775...for Cre recombinase expression in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 199774-...striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862-PHPeB AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre...striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2...striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857-PHPeB AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3...spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru 214856-PHPeB AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3...

...FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression pNIC-CTHF - C-terminal ...vector with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and...remove the stop codon for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing...vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG...display vector with a C-terminal Myc tag pPMW-attB - pUASp plasmid with N-terminal Myc tag and attB for ...adds an N-terminal myristoylation signal GST Protein purification pEBG - N-terminal GST for... N-terminal His6-GST-TEV for bacterial expression pDest-565 - N-terminal ...

...enhancer Viviana Gradinaru 99133-AAV1 pAAV-CAG-fDIO-mNeonGreen An AAV genome with Flp recombinase-dependent ...Cre-dependent recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies... tools and emphasizes their rapid and broad dissemination to the research community. Browse plasmids ,...83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...

...Cre-lox recombination principles, scientists have developed constructs to activate/inactivate genes when...respectively. Split Cre: Cre recombinase is split in half into N and C terminal Cre fragments (NCre and CCre...photo-activatable cre recombinase CAG AAV Bruchas 75268 pAAV-CRY2-CreN photo-activatable cre recombinase CAG AAV Bruchas...pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal split-Cre CMV...pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre C-terminal split-Cre-ERT2...photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F Catalytically inactive PA-Cre CMV...FRT: Alternative recombinase system for the control of gene expression. Flp recombinase recognizes FRT ...

...protein kinase kinase kinase 14 FTDCR1B, HS, HSNIK, NIK MAP3K8 mitogen-activated protein kinase kinase kinase...protein kinase kinase 1 MAPKK1, MEK1, MKK1, PRKMK1 MAP2K2 mitogen-activated protein kinase kinase 2 FLJ26075...Kits and Collections Human Kinase ORF Kit : 558 distinct human kinases and kinase-related protein Open Reading...expression. Myristoylated Kinase Kit : Consists of 190 human kinases and kinase-related open reading frames...phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma...phosphoinositide-3-kinase, regulatory subunit 1 (alpha) GRB1, p85, p85-ALPHA PIK3R2 phosphoinositide-3-kinase, regulatory...plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase receptor CD87, UPAR...

...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular... Cre and Flp (B,E). When the correct combinations of recombinases are present, the introns are excised...expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...intersectional cell population definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional...expressed in cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters...

...inducible vector for expression of a recombinant protein with an N-terminal 6x His tag and an MBP tag. Compatible...inducible vector for expression of a recombinant protein with an N-terminal 6x His tag and an MBP tag. Contains...promoters (Lactose/IPTG inducible) Various combinations of N and C-terminal Strep and His tags Thorben Dammeyer... and without different combinations of His and Strep tags as well as N-terminal PelB tags for localization...be expressed in B. subtilis Various combinations of N and C-terminal Strep, His, MBP, TRX, GST, SUMO, MISTIC...Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN fused to an E. coli codon optimized...Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN fused to an E. coli codon optimized...

...Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated...STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2... CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2 Connector enhancer of kinase suppressor of Ras CYTH2...receptor tyrosine kinase 2 ERK MAPK1 MAPK3 Also known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2...coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor tyrosine kinase RPS6KA RPS6KA1 RPS6KA2... Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1 RPTOR Regulatory associated...within the cell. GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal...

...Flpo recombinase Recombinases Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases...expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases Wilson 105558... expression Recombinases Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases Wilson 55634...mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637... expression Recombinases Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Deisseroth ...expression Recombinases Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Zeng 174378... expression Recombinases Wickersham 183412 pAAV-CAG-FlpO CAG Flpo expression Recombinases Janelia 51085...

... catalyze cytidine deamination instead of (or in addition to) adenosine deamination, producing cytosine...Plasmids: CRISPR transposases CRISPR Recombinases and Integrases Recombinases, and their subfamily integrases...interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have modified for...target sequence present in the gRNA. CRISPR was originally employed to knock out target genes in various...SpCas9, has one active nuclease domain and one inactivated one, allowing it to generate DNA nicks by cutting...cutting only one strand. Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease...backbone HypaCas9 - increase Cas9 proofreading and discrimination evoCas9 - decrease off-target effects xCas9...

...sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia...luciferase reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI ...which was a source of false positives with the original STARR-seq method. Learn more about STARR-seq in...87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale... pLenti6.2-ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival... pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral...87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale...

...displays enhanced transduction of the retina following subretinal administration and enhanced transduction...transduction and lateral spread in the retina following subretinal injection. It was identified by John ...transduction and lateral spread in the retina following subretinal injection as compared to the parental...Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol ...may also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part ...possible) in vivo testing, and full sequencing of the final viral vector preparation. These viral vector preparations...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...

...Cre Recombinase James M. Wilson AV-1-PV2676 105553-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase James... Cre Recombinase James M. Wilson AV-2-PV1090 105537-AAV2 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James...Cre.SV40 Cre Recombinase James M. Wilson AV-5-PV2408 105550-AAV5 pAAV.GFAP.Cre.WPRE.hGH Cre Recombinase James... Cre Recombinase James M. Wilson AV-8-PV1090 105537-AAV8 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James... Cre Recombinase James M. Wilson AV-9-PV1090 105537-AAV9 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 Cre Recombinase James M. Wilson AV-9-PV2394 107788-AAV9 AAV.rTH.PI.Cre.SV40 Cre Recombinase James M...Cre Recombinase James M. Wilson AV-9-PV2676 105553-AAV9 pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase James...

...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for ... to NeuN, plus Cre recombinase 60228 : sgRNA targeted to LacZ, plus Cre recombinase 60229 : sgRNA cloning... backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH 60231...plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding...consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2...targeting efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only...KRAS , p53 , and LKB1 , plus luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for...

...pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...new destination vectors can be used to create TALE-transcription factors and are used at the final assembly...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate TALEN ...-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN kit (Bogdanove...expression vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully...several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available with homodimeric or enhanced...

...the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry... of kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli...111705-8 ) is available from Addgene. Screening kinases. The mode #1 phosphosite library can also be synthesized...enhanced biological tolerance. Mohler K, Moen JM, Rogulina S, Rinehart J. Mol Syst Biol 2023 Aug 8;19(8):...interactions. Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart...7):638-644. PubMed (Link opens in a new window) Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented...

...pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG...light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng ... Engineered systemic constructs from Viviana Gradinaru's lab at Caltech. Viral...engineered systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link...possible) in vivo testing, and full sequencing of the final viral vector preparation. Systemic Delivery AAV ...NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center (Link opens ...CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV....

...viruses (AAV) are small viruses originally discovered as contaminants of adenovirus stocks. One major ...and Cap2 Melina Fan 104964 pAAV2/5 AAV5 AAV packaging plasmid, expressing Rep2 and Cap5 Melina Fan 112863...low immune response and low toxicity. Although recombinant AAV does not integrate into the host genome, ...controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S AAV packaging ...controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging...

...absence of unexpected DNA contaminants. The data is also used to determine recombination rates in FLEX/DIO/fDIO...Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product...mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event...is performed on viral genomes isolated from the final AAV preparation. Sequencing results are analyzed...particularly in large animal studies. Endotoxin contamination is minimized by using an endotoxin-free plasmid.... To minimize the immunogenic properties of the final vector preparation, the quantity of gram-negative...Takara, and is routinely tested for mycoplasma contamination using mycoplasma detection kits. The cell line...

...Verdoes Backbones for mouse recombinant antibodies A panel of recombinant monoclonal antibodies against... Collection Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...alternative approach is to use synthetically produced recombinant antibodies, created by cloning antibody components...Some scientists choose to work with full length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian...research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently...of production due to their small size. These recombinant antibody fragments can be used in experiments...tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep. 2014 Jul 30;4:5885. Andrew...

...enhancer plasmids for fluorescent labeling and recombination across brain regions and populations created...ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population Region 230551 pAAV-AiE2638m-minBG-SYFP2...pAAV-AiE2563m-minBG-SYFP2-WPRE3-BGHpA AiP1969 AiE2563m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2403m-minBG-SYFP2-WPRE3-BGHpA AiP1772 AiE2403m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA AiP2107 AiE2664m SYFP2 Entorhinal area - L3 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-iCre(R297T)-BGHpA AiP20169 AiE2664m Cre Entorhinal area - L3 pyramidal neurons Hippocampal formation...minBG-SYFP2-WPRE3-BGHpA AiP15079 AiE0127m_3xC2 SYFP2 Entorhinal area - L5b neurons Hippocampal formation 230556...

...Antibodies produced this way are called recombinant antibodies. Recombinant antibodies are created by cloning... time and labor intensive. Recombinant expression validation A recombinant expression strategy is used...cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals... of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from plasmids... and can either be used while suspended in the original media or isolated and placed in a buffer. Antibody... binding site. This process, known as V(D)J recombination, allows for a wide variety of antibodies to ...abundant isotype in blood, also found in tissues; predominant isotype in secondary immune response Monomer ...

...growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1 and mTORC2... and multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. ...homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin...S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von ...LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as ...as MAPKAP1; Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin ...; tumor protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB...

...to brainstorm practical strategies for using combinations of AAV-based molecular tools to achieve targeted...Cambridge, MA, 02139 Organizing Committee Chair: Dr. Melina Fan, Co-Founder and CSO, Addgene Organizing Committee...Dr. Constance Cepko, Harvard Medical School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. ...Research Campus Kenton Woodard Penn Vector Core Melina Fan, Karen Guerin, and Leila Haery from Addgene...Friday, September 21 9:00 AM Breakfast and Welcome Melina Fan 9:30 AM Cre and Flpo Katherine Matho 10:15 ...Regulation Ben Deverman 2:00 PM Cre-DOG and Combinatorial Strategies Connie Cepko 2:45 PM Break 3:00 PM...Field Kenton Woodard 4:00 PM Wrap Up & Next Steps Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation...

...backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including...zebrafish research. China Zebrafish Resource Center (CZRC) - A resource center based in China focused on collecting...Tol2 transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS,...vectors for cell-type specific conditional gene inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted...origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish - Bret Pearson...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific biotin...

...CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger ...factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1...PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...to activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...

...BbsI yes, cut, N-terminal S. pyogenes Zhang PX854 62886 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang...BbsI yes, cut, N-terminal S. pyogenes Zhang PX852 62884 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang...activate, N-terminal S. pyogenes Zhang PX856 62888 Mammalian BbsI yes, activate, C-terminal S. pyogenes...assembled into a single T-DNA binary vector of choice through Gateway recombination. Qi Multiplex Conditional...pyogenes GFP Vakoc U6>sgRNA(F+E) 59986 Other/Ciona intestinalis BsaI none S. pyogenes Christiaen p(U6a-BsaI-...Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses...expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning by ...

...Homologous Recombination (HR)? How long should the homology arms be for efficient recombination? The most...Dr. Le Cong, the first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a ...Plasmids Dr. Le Cong, the first author of one of the seminal articles from Dr. Feng Zhang's Lab ( Science , ...to learn more. CRISPR Design FAQs Homologous Recombination (HR) FAQs At the Bench FAQs CRISPR Design FAQs... to express the Cas9n (nickase). Homologous Recombination (HR) FAQs Can you summarize HR template vector...specific mutations or insertions by Homologous Recombination (HR), what is the length of each homology arm...destroy the BbsI sites, a double digest should discriminate between positive and negative clones. Clones...

...Bacterial Expression mCardinal 604 659 17 5.3 27 min Prone to dimerization mCardinal-N1 - Mammalian Expression...Expression mCardinal-C1 - Mammalian Expression mCardinal-pBAD - Bacterial Expression mMaroon 610 652 - Monomer...eGFP pPD95_75 - C-terminal GFP for C. elegans expression pHT101-mCherry - N- or C-terminal mCherry for C....that are used extensively by life scientists. The original green fluorescent protein (GFP) was cloned in ...Davidson Lab Plasmids - Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids...tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal GFP for bacterial expression Davidson Lab Plasmids...Methods, September 2007, Vol. 4 No. 9, pp. 741-6 Shemiakina et al. : Nature Communications, November 2012...

...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Innovation (IPI) Collection! Here, you’ll find unique recombinant antibodies targeting families of cell surface...window) and an optimized sorting process. This combination allows IPI to generate antibodies to highly conserved...courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that recognize common ... tags and other common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended...

...anti-kinesin recombinant scFvs . Niwa et al. Cytoskeleton. 2023 Apr 10. Explore the recombinant monoclonal... upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads ...familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic mutations...Huntington’s disease (HD) is a fatal autosomal dominant genetic disorder that causes the progressive degeneration... gene and late-onset AD. It is more likely a combination of gene variation and lifestyle or environmental...Saha et al. Nat Commun. 2023 Feb 2. Study the C terminal domain of TDP-43 to better understand its function... tools and emphasizes their rapid and broad dissemination to the research community. This collection highlights...

...methods and quality control processes for purified recombinant antibodies....webpage for further information. Production For recombinant antibodies (rAb) encoded by mammalian expression... buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline ...sample is adjusted by diluting or concentrating to a final concentration of 0.9–1.1 mg/mL. Antibody concentration...software and a standard curve is generated. The final antibody concentration is calculated from the standard...performed in parallel with a previous lot of recombinant antibody or the hybridoma-derived equivalent ... Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified...

...) N-terminal Anti-MECP2 (M7443) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2...syndrome can be inherited. In females, random X inactivation results in approximately half of cells expressing...expressing mutant MECP2 . Skewing of these X inactivation ratios can affect disease severity. The MECP2...several functional domains, including: the N - t erminal D omain (NTD) the M ethyl B inding D omain (MBD...signal (not essential for localization) the C - t erminal D omain (CTD) The most common missense mutations...antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling...direction and degree of skewing of X-chromosome inactivation. J Med Genet . 44, 148–152. (Link opens in a...

...3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina N/A 1 Varies ...Human Ebert 3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR KO library 174592 Knockout...contained in the library represent all 4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-wide... Human Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 ... 84,963 Human CRISPR Knockout Pooled Library (Gattinara) 136986 Knockout Human Doench 3rd 2 40,964 Ingolia...Library 140633 Knockout Human Wei 3rd 3 58,630 Human Kinase Domain-Focused CRISPR Knockout Library 117725 Knockout...10 8,158 TUNEYALI Library 217744 Knockout Yeast Borodina N/A 1 56 Turner Lab Human messenger-RBP sgRNA ...

...and shRNA against p53 in different gene/insert combinations A more efficient method to generate integration-free...NR5A2, and microRNA 302/367 in three different combinations of reprogramming factors Efficient germ-line...40. Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS... and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells induced Beta Cells Adenoviral Mouse Long-...pancreatic beta cells generated by lineage conversion of acinar cells. Nature Biotechnology. 2014 Dec;32(12):1223... of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):...

...or C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging...deposited a plasmid which introduces an N- or C- terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes...untagged SpCas9 (Addgene 41815 or #44719 ) in combination with gRNA_AAVS1-T2 (Addgene #41818) or using ...modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in the following articles...selection markers are available. An improved FLP recombinase expression vector allows for efficient marker...

...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...990 KB) Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2...Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or ...Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification...

...Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter...feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible...feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible...Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru Tools for Affinity Purification These AAV encode...tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby... and synaptophysin-mRuby for labeling of axon terminals. 1 Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato...