MultiGreen: A multiplexing architecture for GreenGate cloning.

Pennetti VJ, LaFayette PR, Parrott WA
PLoS One. 2024 Sep 18;19(9):e0306008. doi: 10.1371/journal.pone.0306008. eCollection 2024. PubMed Article

These materials are included in the MultiGreen Kit.

Plasmids from Article

ID	Plasmid	Purpose
222027	pGGPK-AG2	Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG.
222028	pVP076	Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
222029	pVP077	Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
222030	pVP078	MultiGreen 2.0 AB level 1 acceptor vector, spectinomycin selection.
222031	pVP079	MultiGreen 2.0 BC level 1 acceptor vector, spectinomycin selection.
222032	pVP080	MultiGreen 2.0 CD level 1 acceptor vector, spectinomycin selection.
222033	pVP081	MultiGreen 2.0 DE level 1 acceptor vector, spectinomycin selection.
222034	pVP082	MultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.
222035	pVP083	MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.
222036	pVP084	meffBlue chromoprotein in a MultiGreen 1.0 HG entry vector.
222037	pVP085	spisPink chromoprotein in a MultiGreen 1.0 HG entry vector.
222038	pVP086	mRFP1e chromoprotein in a MultiGreen 1.0 HG entry vector.
222039	pVP088	MultiGreen 1.0 HG entry vector containing filler sequence.
222040	pVP089	MultiGreen 1.0 base HG entry vector.
222041	pVP090	MultiGreen 1.0 FH entry vector containing filler sequence.
222042	pVP091	MultiGreen 1.0 base FH entry vector.
222043	pVP096	pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.
222044	pVP333	GreenGate AB bridge module containing filler sequence.
222045	pVP334	GreenGate FG bridge module containing filler sequence.
222046	pVP120	GreenGate BG bridge module containing filler sequence.
222047	pVP121	GreenGate CG bridge module containing filler sequence.
222048	pVP122	GreenGate DG bridge module containing filler sequence.
222049	pVP123	GreenGate EG bridge module containing filler sequence.
222050	pVP183	GreenGate DF bridge module containing filler sequence.
222051	pVP184	GreenGate EF bridge module containing filler sequence.
222052	pVP185	GreenGate BF bridge module containing filler sequence.
222053	pVP186	GreenGate CF bridge module containing filler sequence.
222054	pVP224	GreenGate AC bridge module containing filler sequence.
222055	pVP225	GreenGate AD bridge module containing filler sequence.
222056	pVP226	GreenGate AE bridge module containing filler sequence.
222057	pVP227	GreenGate AF bridge module containing filller sequence.
222058	pVP228	GreenGate BD bridge module containing filler sequence.
222059	pVP229	GreenGate BE bridge module containing filler sequence.
222060	pVP230	GreenGate CD bridge module containing filler sequence.
222061	pVP231	GreenGate CE bridge module containing filler sequence.
222062	pVP293	GreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.
222063	pVP294	GreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.
222064	pVP295	GreenGate AB base entry vector with an Esp3I domesticated backbone.
222065	pVP296	GreenGate BC base entry vector with an Esp3I domesticated backbone.
222066	pVP297	GreenGate CD base entry vector with an Esp3I domesticated backbone.
222067	pVP298	GreenGate DE base entry vector with an Esp3I domesticated backbone.
222068	pVP299	GreenGate EF base entry vector with an Esp3I domesticated backbone.
222069	pVP300	GreenGate FG base entry vector with an Esp3I domesticated backbone.
222070	pVP311	MultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.
222071	pVP312	MultiGreen 2.0 BC level 1 acceptor vector, kanamycin selection.
222072	pVP313	MultiGreen 2.0 CD level 1 acceptor vector, kanamycin selection.
222073	pVP314	MultiGreen 2.0 DE level 1 acceptor vector, kanamycin selection.
222074	pVP315	MultiGreen 2.0 EF level 1 acceptor vector, kanamycin selection.
222075	pVP316	MultiGreen 2.0 FG level 1 acceptor vector, kanamycin selection.