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We narrowed to 39,290 results for: NAM

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Showing: 5051 - 5100 of 39290 results

  1. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Article
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Insert
    Histone H2B - Citrine (H2BC21 Human, Synthetic)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    5xTetO pEF alpha
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179436
    Purpose
    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    HS4-TRE-cit-HS4-mch (DH)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179436-plasmid-map-sequence-id-355459

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  5. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

    Plasmid
    #100716
    Purpose
    B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting ATP1A1 (cloned using BsmBI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available Since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100716-plasmid-map-sequence-id-193652

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  14. Epac-S-H159

    Plasmid
    #170347
    Purpose
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170347-plasmid-map-sequence-id-336136

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  16. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179437
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179437-plasmid-map-sequence-id-355553

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  19. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Article
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

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  21. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101851-plasmid-map-sequence-id-200251

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  22. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  24. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid
    #122003
    Purpose
    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast
    Depositor
    Brian Kuhlman
    Article
    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d
    Insert
    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mVenus-FLAG
    Expression
    Yeast
    Mutation
    Set2 point mutations K538G, K539S, R549G, K550S, …
    Promoter
    ADH1
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    122003-plasmid-map-sequence-id-238611

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  28. pX462-ABE8e-nSaCas9

    Plasmid
    #237462
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SaCas9 -6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237462-plasmid-map-sequence-id-476147

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  29. pCW-MRTFA-HA

    Plasmid
    #247436
    Purpose
    siRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    MRTF-A (Mrtfa Mouse)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    siRNA resistant - no amino acid mutations
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247436-plasmid-map-sequence-id-491537

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  30. pCW-Rho(G14V)-HA

    Plasmid
    #247437
    Purpose
    HA-tagged human RhoA(G14V) cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    RhoA(G14V) (RHOA Human)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G14V mutation (constitutive active mutant)
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247437-plasmid-map-sequence-id-490945

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  39. pcDNA3.1-PDGFRA-R487L-V5/HIS

    Plasmid
    #234761
    Purpose
    Expression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal Tumor
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    R487L substitution
    Promoter
    CMV
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234761-plasmid-map-sequence-id-468953

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  41. pcDNA3.1-PDGFRA-T674I-V5/HIS

    Plasmid
    #234756
    Purpose
    Expression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinib
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    T674I substitution
    Promoter
    CMV
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    234756-plasmid-map-sequence-id-468865

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  43. pMVP-SEC24C-G220C

    Plasmid
    #231560
    Purpose
    pMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (G220C) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    G220C, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231560-plasmid-map-sequence-id-460046

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  44. pMVP-SEC24C-S107F

    Plasmid
    #231561
    Purpose
    pMVP expression vector for human SEC24C (S107F mutation, closesd, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (S107F) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    S107F, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231561-plasmid-map-sequence-id-459737

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Showing: 5051 - 5100 of 39290 results