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174,957 results

Showing: 2061 - 2080 of 174957 results

  1. ABE9-SpRY

    Plasmid
    #242976
    Purpose
    A-to-G base editor fused to PAM-flexible SpRY-Cas9 nickase.
    Depositor
    Marc Freichel
    Article
    Ong et al Sci Rep. 2026 Feb 20;16(1):7463. doi: 10
    Insert
    pCMV-TadA-8e (N108Q/L145T)-nSpRYCas9
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    6*His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    242976-plasmid-map-sequence-id-486100

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  5. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  12. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  13. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

    Plasmid
    #44361
    Purpose
    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Promoter
    human Synapsin 1
    Available Since
    May 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44361-plasmid-map-sequence-id-148598

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  17. pENN.AAV.CamKII 0.4.Cre.SV40

    Plasmid
    #105558
    Purpose
    AAV expression of Cre from CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CamKII
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105558-plasmid-map-sequence-id-202399

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  20. pKM512

    Plasmid
    #140192
    Purpose
    For curing of Orbit-integrated plasmids and associated drug marker
    Depositor
    Kenan Murphy
    Article
    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
    Inserts
    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    Expression
    Bacterial
    Mutation
    Cloned in gp47 directinality factor in place of R…
    Promoter
    EM7 and Ptet
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    140192-plasmid-map-sequence-id-418051

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Showing: 2061 - 2080 of 174957 results