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T7-LacO-LgBiT

Plasmid

#216779

Purpose

Expresses LgBiT in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

LgBiT

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAP03-acc(3)IV

Plasmid

#69862

Purpose

pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

Depositor

Bradley Moore

Article

Tang et al ACS Chem Biol. 2015 Oct 21.

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAP-BAC

Plasmid

#120229

Purpose

TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Expression

Bacterial

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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bZiPro

Plasmid

#86846

Purpose

express NS2B-NS3 protease in E.coli

Depositor

Dahai Luo

Article

Zhang et al Science. 2016 Dec 8. pii: aai9309.

Inserts

NS3 1-177
NS2B45-96

Tags

a hexahistidine tag and a thrombin cleavage site

Expression

Bacterial

Mutation

NS2B cofactor region (residues 45–96) and NS3 pro…

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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GAL4BD-VP16/pDON207

Plasmid

#201230

Purpose

GAL4-VP16 transcription factor

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

GAL4BD-VP16

Expression

Plant

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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PiggyBac-FLAG-hTET2-IRES-Blast

Plasmid

#239819

Purpose

PiggyBac vector to express full length human TET2 with 2X N-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Knatko et al Blood Adv. 2025 Jun 24:bloodadvances.202

Insert

FLAG-tagged human TET2

Tags

2x FLAG tag on TET2

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-CreiLOV

Plasmid

#192817

Purpose

Expresses CreiLOV in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pUGI-NLS

Plasmid

#101091

Purpose

Expresses UGI in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

UGI

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b N17

Plasmid

#118322

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b N17 (RAP1B Bovine)

Tags

Flag

Expression

Mammalian

Mutation

S17N

Promoter

CMV

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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DL146_plenti_TRE_GFP-iPAK4

Plasmid

#187446

Purpose

Tet-On inducible expression of eGFP-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box-Pak4

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GCaMP6s

Plasmid

#166606

Purpose

Encodes Cre-dependent GCaMP6s under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GCaMP6s

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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TEV Protease-mCherry

Plasmid

#243793

Purpose

Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TEV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2(1-10)

Plasmid

#82603

Purpose

Lentiviral vector for constitutive expression of sfCherry2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

SFFV

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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miniCMV-EGFP-tDeg

Plasmid

#185400

Purpose

EGFP-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

EGFP-tDeg

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP1

Plasmid

#188896

Purpose

Express 6xHis-Tag miniGFP1 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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epB_UbC_CymRV5-nls-GFP_DEx2

Plasmid

#119906

Purpose

ePiggyBac mammalian expression plasmid for CymR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRSVBneoT

Plasmid

#22883

Depositor

James Pipas

Article

Srinivasan et al Mol Cell Biol. 1997 Aug . 17(8):4761-73.

Insert

Early region SV40

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP_TOMM20_sfCherry2(11)

Plasmid

#83033

Purpose

Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

TOMM20_sfCherry+11

Expression

Mammalian

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Tet-on circRNA Expression Vector

Plasmid

#92351

Purpose

The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

Depositor

Ling-Ling Chen

Article

Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

Insert

mCherry and split EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

pTet

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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MSCB-hDot1Lwt

Plasmid

#74173

Purpose

For packaging hDot1Lwt to retrovirus.

Depositor

Yi Zhang

Article

Okada et al Cell. 2005 Apr 22;121(2):167-78.

Insert

hDot1L (DOT1L Human)

Use

Retroviral

Tags

HA

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GRvT

Plasmid

#87363

Purpose

Expresses GRvT, a tandem heterodimer green and red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GRvT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_SpG‐ABE8e(V106W)

Plasmid

#187069

Purpose

ABE8e(V106W)-SpG

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

SpG‐ABE8e(V106W)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-NanoLuc

Plasmid

#188247

Purpose

Template plasmid to transcribe mRNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCold CL7-Cas9

Plasmid

#124890

Purpose

obtaining full Cas9 RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas9

Expression

Bacterial

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-mcs2

Plasmid

#138362

Purpose

Gateway compatible 5' entry clone contains mutli-cloning sites sequence2.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-IQGAP1

Plasmid

#30118

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP2

Plasmid

#188897

Purpose

Express 6xHis-Tag miniGFP2 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-RRN3/TIF1A

Plasmid

#17661

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RRN3/TIF1A (RRN3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

See depositor comments below

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-fDIO-tTA

Plasmid

#166597

Purpose

Encodes Flp-dependent tTA

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

tTA

Use

AAV

Promoter

human synapsin 1

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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HRV-B3 IRES

Plasmid

#189969

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

HRV-B3 IRES

Use

Cloning

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGH156_MS2-AID-Hygro

Plasmid

#85415

Purpose

lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-RRvT

Plasmid

#87364

Purpose

Expresses RRvT, a tandem heterodimer red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

RRvT

Tags

His-Tag

Expression

Bacterial

Promoter

pBad (Arabinose)

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pOsU3

Plasmid

#170132

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1802_pmax-RBFOX1N-dCasRx-C

Plasmid

#118635

Purpose

Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-dCasRx-C

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCold CL7-Cas12a

Plasmid

#124891

Purpose

obtaining full Cas12a RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas12a

Expression

Bacterial

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG2AB-eIF3klefhdm

Plasmid

#240041

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d and eIF3m expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m

Tags

eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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3xFLAG-NanoLuc

Plasmid

#189975

Purpose

Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Cloning

Tags

3xFLAG

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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