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We narrowed to 5,794 results for: RIK

Showing: 4121 - 4160 of 5794 results

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PB-hSyn-mSyp-RT1-24xGCN4

Plasmid

#106300

Purpose

The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

mSyp-RT1-24xGCN4 (Syp Mouse)

Use

Tags

24xGCN4

Expression

Mammalian

Mutation

Promoter

human Synapsin1

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

Plasmid

#85750

Purpose

Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type II-C anti-CRISPR AcrIIC3Nme
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-SEC61B-mEGFP

Plasmid

#115921

Purpose

donor to insert mEGFP at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-mEGFP

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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His-Cavin1-GFP

Plasmid

#170859

Purpose

Mammalian expression of His-Cavin1-GFP.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

caveolae associated 1 (Cavin1) (Cavin1 Mouse)

Use

Tags

(His)6 and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR/U6 HDAC4 shRNA

Plasmid

#32220

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against murine HDAC4 (Hdac4 Mouse)

Use

RNAi; Gateway u6 entry vector

Tags

Expression

Mutation

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
pBacPAK8-His-myc-Rbx1

Plasmid

#29506

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Rbx1 (Rbx1 Mouse)

Use

Tags

His and myc

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
Gal4 PGC1 alpha 1-350

Plasmid

#8893

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Tags

Gal4 DBD

Expression

Mammalian

Mutation

amino acids 1-350 of PGC-1 alpha

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
Sema5a-AP-His

Plasmid

#72035

Purpose

Expresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema5a (Sema5a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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hsDCX-pHAT

Plasmid

#83928

Purpose

hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

human Doublecortin isoform b (DCX Human)

Use

Tags

6-His tag and Strep-TagII

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pfastbacHTb/mClock26-384

Plasmid

#47336

Purpose

mClock fragment cloned into pfastbacHTb vector with Hexa His tag

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

His and Tev

Expression

Mutation

aa 26-384

Promoter

PH

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED6 (Med6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
CKAP2-mNeonGreen pHAT

Plasmid

#175947

Purpose

Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Susanne Bechstedt

Article

McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

Insert

CKAP2 (Ckap2 Mouse)

Use

Tags

6His, StrepTagII, and mNeonGreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Use

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-DM-eGFP-KDEL

Plasmid

#177728

Purpose

DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFlucDM

Use

Tags

KDEL, Prolactin Signal Sequence, and eGFP

Expression

Bacterial and Mammalian

Mutation

R188Q, R261Q (Destabilized Mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBS/U6/RNAi gamma-tubulin shRNA mouse

Plasmid

#70666

Purpose

Reduced the expression of gamma-tubulin in murine cells

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)

Use

U6 promoter

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-IC2-N106

Plasmid

#51411

Purpose

expression of GFP-tagged N-terminal AA 1-106 of dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

Plasmid

#105025

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

Plasmid

#105026

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

Plasmid

#161993

Purpose

Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (Snx9 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

R286A Y287A K288A

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PGC1(1-180)

Plasmid

#1028

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains only aa1-180 (the EcoRI site)

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
mCherry-C1 SNX9 KR mutant K267N R327N

Plasmid

#161994

Purpose

Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (Snx9 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

K267N R327N

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-181a1-Dusp6

Plasmid

#17849

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

Dusp6-EGFP (Dusp6 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C192A-Myc

Plasmid

#201168

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETa-mEos4b-V69T

Plasmid

#98572

Purpose

photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos4b-V69T

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-HA-GFP

Plasmid

#120903

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Plxnc1-Fc-His

Plasmid

#72131

Purpose

Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnc1 (Plxnc1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(K271Q)

Plasmid

#133270

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Tags

Expression

Mutation

K271Q

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Use

Tags

Expression

Mutation

Q258K

Promoter

T7

Available since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa2-A69T

Plasmid

#98578

Purpose

photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

pcDronpa2-A69T

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV V5-nurim

Plasmid

#175110

Purpose

Lentiviral expression of V5-tagged mouse Nrm

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Nrm (Nrm Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Sema3c(L)-Fc-His

Plasmid

#72141

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-mIFITM3-K24,83,88,104A

Plasmid

#58408

Purpose

Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pBS-f1(-)-GAPDH-IRES-dTomato

Plasmid

#115919

Purpose

donor to insert IRES-dTomato at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-dTomato

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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mOC-STAMP_3xFlag-Myc-CMV26

Plasmid

#73577

Purpose

Express mouse OC-STAMP in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Tags

3x Flag and c-Myc tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Txnip

Plasmid

#178437

Purpose

doxycycline-inducible expression of Mouse Txnip in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Txnip (Txnip Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Trpc2-IRES-taumCherry-ACNF TV

Plasmid

#105212

Purpose

targeting vector to generate a Trpc2-IRES-taumCherry mouse strain.

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Trpc2-IRES-taumCherry-ACNF (Trpc2 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA-CAML-Myc-His

Plasmid

#22295

Depositor

Erik Lillehoj

Article

Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

Insert

calcium modulating ligand (CAMLG Human)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2009

Availability

Academic Institutions and Nonprofits only
Sema3c(S)-Fc-His

Plasmid

#72142

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS540-pCSDest2-AcrIIC4Hpa

Plasmid

#113434

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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