166,818 results

1. pCMV5-Flag-HDAC3

   Plasmid

   #63676

   Purpose

   Mammalian expression of Flag tagged HDAC3

   Depositor

   Lingfang Zeng, Qingbo Xu

   Article

   Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

   Insert

   HDAC3 (Hdac3 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

2. MELOPS

   Plasmid

   #80151

   Purpose

   Genetically encoded melanosome-localized pH sensor.

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

   Insert

   OCA2 (OCA2 Human)
   

   Use

   Tags

   mNectarine in first luminal loop

   Expression

   Mammalian

   Mutation

   nonpathologic polymorphism D257A, pore mutation V…

   Promoter

   CMV

   Available Since

   Sept. 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

3. MoClo Plant Parts Kit

   Plasmid Kit

   #1000000047

   Purpose

   Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Available Since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAGD248

   Plasmid

   #219498

   Purpose

   Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlagCMV2-UbcH8

   Plasmid

   #12442

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   UbcH8 (UBE2L6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204341

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-i3/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. HyperNova/pRSETB

   Plasmid

   #221456

   Purpose

   Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Kiwamu Takemoto

   Article

   Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

   Insert

   HyperNova
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRosa26-EF1a-hCas9IRESneo

   Plasmid

   #67987

   Purpose

   Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-hCas9IRESneopA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMaCTag-P11

   Plasmid

   #120022

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mScarlet-i
   

   Use

   Pcr template

   Tags

   mScarlet-i

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3 WT B-Myb

    Plasmid

    #25965

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

    Insert

    B-Myb (MYBL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDAC565

    Plasmid

    #195242

    Purpose

    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDUET-1_VNp-anti-GFP mAb

    Plasmid

    #214955

    Purpose

    Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-GFP mAb heavy chain
    VNp-anti-GFP mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP955

    Plasmid

    #98784

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-TRPV1exCellHalo

    Plasmid

    #220307

    Purpose

    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

    Depositor

    Eric Senning

    Article

    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

    Insert

    rTRPV1exCellHalo (Trpv1 Rat)
    

    Use

    Tags

    circularly permutated Halotag (Deo et al., Nat Ch…

    Expression

    Mammalian

    Mutation

    linker included to insert cpHaloTag (

    Promoter

    CMV promoter

    Available Since

    July 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. TFORF3054

    Plasmid

    #144530

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SALL4 (SALL4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. nLbCas12a

    Plasmid

    #213745

    Purpose

    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    nLbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dLight1.3b

    Plasmid

    #111056

    Purpose

    Expresses dLight1.3b in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3b
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge