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pJH1498
Plasmid#178600PurposePrgef-1 INS-18::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-18 fused to GFPDepositorInsertINS-18 (ins-18 Nematode)
UseTagsExpressionWormMutationPromoterPrgef-1Available sinceJan. 5, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
GP821
Plasmid#164552PurposeExpresses Smo-FLAG K434RDepositorInsertSmoothened K434R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationOne of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP823
Plasmid#164554PurposeExpresses Smo-FLAG K448RDepositorInsertSmoothened K448R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationOne of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP822
Plasmid#164553PurposeExpresses Smo-FLAG K444RDepositorInsertSmoothened K444R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationOne of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoteract1Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr_2pabPAC
Plasmid#158971Purpose2P-activatable bPAC for lentivirusDepositorInsert2pabPAC
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW294
Plasmid#139491PurposeInsertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGRE2 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGRE2Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW295
Plasmid#139492PurposeInsertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpALD3 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpALD3Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP266
Plasmid#139488PurposeInsertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpCTT1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpCTT1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP264
Plasmid#139487PurposeInsertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP566
Plasmid#139500PurposeGene tagging vector to add mCherry Tag and membrane anchoring motifDepositorInsertmCherry-CaaX
UseTagsExpressionYeastMutationWTPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW200
Plasmid#139489PurposeInsertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGPD1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGPD1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW293
Plasmid#139490PurposeInsertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpHSP12 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpHSP12Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
UseTagsmCherryExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only