We narrowed to 8,021 results for: gal

1. pOAD eIF4G1 (1-341)

   Plasmid

   #196833

   Purpose

   Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   eIF4G1 (TIF4631 Budding Yeast)
   

   Use

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   amino acids 1-341

   Promoter

   ADH

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pACT2-μ1A

   Plasmid

   #198168

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-1 μ1A
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOBD2 Pab1 WT (123-204)

   Plasmid

   #196834

   Purpose

   Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   Pab1 (PAB1 Budding Yeast)
   

   Use

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   123-204 amino acids

   Promoter

   ADH

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOBD2 Pab1 E181R (123-204)

   Plasmid

   #196835

   Purpose

   Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   Pab1 (PAB1 Budding Yeast)
   

   Use

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   123-204 amino acids & E181R

   Promoter

   ADH

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBT9-tepsin

   Plasmid

   #196647

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   tepsin (TEPSIN Human)
   

   Use

   Yeast two-hybrid vector

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   silent substitution in codon 199 to eliminate int…

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR_PGK_SNIPR_(GGS)3_SNIPR

   Plasmid

   #188343

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)3, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_(GGS)3_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR_PGK_SNIPR_(GGS)9_SNIPR

   Plasmid

   #188345

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)9, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_(GGS)9_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR_PGK_SNIPR_(GGS)12_SNIPR

   Plasmid

   #188346

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)12, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_(GGS)12_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR_PGK_SNIPR_(GGS)15_SNIPR

   Plasmid

   #188347

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)15, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_(GGS)15_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR_PGK_SNIPR_(GGS)18_SNIPR

    Plasmid

    #188348

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)18, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_(GGS)18_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR_PGK_SNIPR_3xFLAG-tag tether

    Plasmid

    #188352

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a 3X-FLAG-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_3X-FLAG-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR_PGK_SNIPR_Notch2 del-NRR

    Plasmid

    #188354

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch2-del-NRR-ECD, a Notch2-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Notch2-NRRdeletion-ECD_Notch2-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_PGK_SNIPR_Notch3 del-NRR

    Plasmid

    #188355

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-del-NRR-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Notch3-NRRdeletion-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR_PGK_SNIPR_IgG4 SNIPR

    Plasmid

    #188359

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a IgG4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_IgG4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR_PGK_SNIPR_OX40 SNIPR

    Plasmid

    #188360

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a OX40-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_OX40-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR_PGK_SNIPR_CD8alpha variant 3

    Plasmid

    #188363

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant3-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR_PGK_SNIPR_CD8alpha variant 2, V319A

    Plasmid

    #188366

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_V319A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_V319A_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSF-p15A-T7- YFP

    Plasmid

    #186568

    Purpose

    p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Linked Free NES (M)

    Plasmid

    #182436

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    FPPS(M)-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-MUC2 D1

    Plasmid

    #181812

    Purpose

    Expresses human MUC2 D1, residues 21-389

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc2 D1 (MUC2 Human)
    

    Use

    Tags

    6xHistadine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pILGFP10CF72 (B11)

    Plasmid

    #185859

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR-ZmIAA29 (yeast codon optimized)

    Plasmid

    #188441

    Purpose

    Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Repressor IAA29 (yeast codon optimized)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. rtTA-sg-CMV152

    Plasmid

    #188485

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. rtTA-sg-CMV162

    Plasmid

    #188486

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

    Plasmid

    #186418

    Purpose

    Expression of scrambled shRNA used as a control for knock down experiments

    Depositor

    Mary Kay Lobo

    Article

    Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

    Insert

    ScramblemiR
    

    Use

    AAV

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    human Synapsin1

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

    Plasmid

    #186419

    Purpose

    Expression of shRNA for the knock down of mouse Egr3

    Depositor

    Mary Kay Lobo

    Article

    Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

    Insert

    miREgr3-5D
    

    Use

    AAV

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    human Synapsin1

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KBB438

    Plasmid

    #185092

    Purpose

    GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Rfa1 with amino acids 338-343 deleted

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX459-sg-CMV152

    Plasmid

    #179916

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX459-sg-CMV51

    Plasmid

    #179915

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX459-sg-CMV162

    Plasmid

    #179917

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGB-Co-NF-kB

    Plasmid

    #180206

    Purpose

    Expression GAL4-fusion of Co-NF-kB in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Use

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-1224

    Promoter

    AdhI

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. gMUM2-Citrine

    Plasmid

    #181965

    Purpose

    MUM2 protein fused to Citrine, under control of native promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    MUM2 (MUM2 Mustard Weed)
    

    Use

    Tags

    Citrine YFP

    Expression

    Plant

    Mutation

    Promoter

    MUM2

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMJB1-RPL1B-Cl

    Plasmid

    #160430

    Purpose

    Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPL1B
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to vary nucleoti…

    Promoter

    S. cerevisiae GAL1

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA4/TO-mu24 1-181-2xStrep

    Plasmid

    #138444

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-181

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA4/TO-mu24 1-191-2xStrep

    Plasmid

    #138445

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-191

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA4/TO-mu24 1-216-2xStrep

    Plasmid

    #138446

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-216

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA4/TO-mu24-2xStrep

    Plasmid

    #138435

    Purpose

    Expresses full-length mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pC0.007

    Plasmid

    #119671

    Purpose

    Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Pcpc560-Dx2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 89 T to C, bp 187 C to A, Duplicated downstrea…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pC0.006

    Plasmid

    #119670

    Purpose

    Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Pcpc560-Ux2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 89 T to C, bp 187 C to A, Duplicated upstream …

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid

    #120250

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

    Plasmid

    #104462

    Purpose

    Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

    Insert

    mCherry-subT391A-FHA1-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBW106

    Plasmid

    #111138

    Purpose

    For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

    Depositor

    Alexander Varshavsky

    Article

    Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

    Insert

    MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
    

    Use

    Tags

    3x Flag

    Expression

    Yeast

    Mutation

    Leu2 deletion

    Promoter

    Gal1

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. LLhE_AW-pHG165

    Plasmid

    #90062

    Purpose

    Constitutive expression of a chimeric LacI:CelR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhE_AW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I48V/Q55A/Q60R

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LLhF-pHG165a

    Plasmid

    #90041

    Purpose

    Constitutive expression of a chimeric LacI:FruR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LLhT-pHG165a

    Plasmid

    #90042

    Purpose

    Constitutive expression of a chimeric LacI:TreR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LLhR-pHG165a

    Plasmid

    #90043

    Purpose

    Constitutive expression of a chimeric LacI:RbsR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. LLhA-pHG165a

    Plasmid

    #90044

    Purpose

    Constitutive expression of a chimeric LacI:AscG transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. LLhE-pHG165

    Plasmid

    #90045

    Purpose

    Constitutive expression of a chimeric LacI:CelR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. LLhC-pHG165a

    Plasmid

    #90046

    Purpose

    Constitutive expression of a chimeric LacI:CytR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge