We narrowed to 26,115 results for: Nes;
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Plasmid#127384PurposeExpresses Cas9 at medium levelDepositorInsertuORF(M)-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRB14
Plasmid#52522Purposeexpresses a myc-tagged version of hCas9 in DrosophilaDepositorInsertS. pyogenes cas9 with humanized codon bias
TagsmycExpressionInsectPromotertubulinAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-GFP-P2A-NptII-9
Plasmid#197741PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEGFP-P2A-NptII bicistronic CDS
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:DsRed:Tnos (GB0361)
Plasmid#68220PurposeTranscriptional unit for DsRed plant expression driven by the 35S promoterDepositorInsertDsRed
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsEYFP and SNAP26mExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-erHRP(N175S mutant)
Plasmid#79909PurposeerHRP(N175S mutant) pCMV backboneDepositorInserterHRP(N175S mutant)
TagsHA tagExpressionMammalianMutationN175S mutationPromoterCMVAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUB7
Plasmid#169698PurposeTemplate plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags6xHis tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSU315
Plasmid#169697PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags1xHA tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABECD
Plasmid#66536PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoterDepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyExpressionBacterialPromoterMutant T7 Promoter - G6Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NFAT5
Plasmid#79506PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDSDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGrRenilla
Plasmid#118060PurposeGBPart - GreenRenilla Luciferase CDSDepositorInsertGreen Renilla
UseLuciferase and Synthetic Biology; GbpartAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Pds1(434)
Plasmid#39847DepositorAvailable SinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVAX_CBI_AcrIIA4
Plasmid#136662PurposeExpresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sitesDepositorInsertAcrIIA4
UseSynthetic BiologyTagsNo tag, No NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pbGH
Plasmid#118061PurposeGBPart - Bovine growth hormone terminatorDepositorInsertbgH_poly(A)
UseSynthetic Biology; GbpartAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
EE-BE4max
Plasmid#138158PurposeC-to-T base editorDepositorInsertrAPOBEC1(EE)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 R126E + R132E; within Cas9 D10APromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pcDNA3
Plasmid#67661Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
ExpressionMammalianAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
c3GIC9n
Plasmid#62192Purposecol1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettesDepositorInserthumanized S. Pyogenes Cas9 (D10A)
UseCRISPRTagsHAMutationD10A substitution (Cas9n)PromoterTRE3GAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV Ef1a DIO GeNL_SS hGtACR1
Plasmid#233511PurposeLuciferase opsin fusion protein (luminopsin) for bioluminescent optogeneticsDepositorInsertsmNeonGreen
ISN-IE
hGtACR1
UseAAVTagsGaussia luciferase signal sequenceAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern/pcDNA3
Plasmid#67660Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorInsertCyan Nano-lantern
ExpressionMammalianAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNLuc
Plasmid#118058PurposeGBPart - NLuc Luciferase CDSDepositorInsertNano Luciferase
UseLuciferase and Synthetic Biology; GbpartAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
ExpressionMammalianAvailable SinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHygromycin (GB0211)
Plasmid#68204PurposeProvides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid partDepositorInsertHygromycin resistance gene coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-H2B-6
Plasmid#55421PurposeLocalization: Nucleus/Histones, Excitation: 433, Emission: 475DepositorInsertH2B (H2BC11 Human)
TagsmCerulean3ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJSK418
Plasmid#205131PurposeExpression of GFP-2xFYVE in DictyosteliumDepositorAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVAX_CBI_AcrIIA2
Plasmid#136661PurposeExpresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sitesDepositorInsertAcrIIA2
UseSynthetic BiologyTagsNo tag, No NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBI-T2A-caMEK
Plasmid#199578PurposeExpresses tKiMBI and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK
UseAAVExpressionMammalianPromoterCMVAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDragon-empty
Plasmid#155018PurposeEmpty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.DepositorTypeEmpty backboneUseMouse TargetingAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:GLuc:bgH
Plasmid#135294PurposeTranscriptional unit for constitutive expression of GLuc in mammalian cellsDepositorInsertCMV:GLuc:bGH
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLuciferase (GB0096)
Plasmid#68201PurposeProvides the CDS of the firefly luciferase protein as a level 0 GoldenBraid partDepositorInsertLuciferase coding region
UseLuciferase and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJG85
Plasmid#89281PurposeEntry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sitesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
TB:7xSMAD_RE:MiniP
Plasmid#133879Purpose7 copies the SMAD DNA binding motifDepositorInsert7 copies the SMAD DNA binding motif
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shTGFBR3 puro
Plasmid#58696PurposeLentiviral shRNA vector for knockdown of human TGFBR3DepositorAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB3035(X-4 MarkerFree)
Plasmid#73273PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-plasma membrane/pcDNA3
Plasmid#51975Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membraneDepositorInsertNano-lantern
TagsHIS and LynExpressionMammalianMutationS257G in Rluc8Available SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pRSETB
Plasmid#67659Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
Tags6xHisExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only