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166,818 results

Showing: 2161 - 2180 of 166818 results

  2. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87828
    Purpose
    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Use
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87828-plasmid-map-sequence-id-175012

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  4. NPY2R-Tango

    Plasmid
    #66454
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    NPY2R (NPY2R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pHeEf1a-mEGFP-NLS

    Plasmid
    #205012
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205012-plasmid-map-sequence-id-405256

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  9. pIND-STAT1-V5

    Plasmid
    #11618
    Depositor
    Steven Johnson
    Insert
    Signal transducer and activator of transcription 1 (STAT1 Human)
    Use
    Ecdysone inducible
    Tags
    V5
    Expression
    Mammalian
    Mutation
    no mutations
    Promoter
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  16. pDS240

    Plasmid
    #34966
    Purpose
    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).
    Depositor
    Michael Glotzer
    Article
    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm
    Insert
    Mid2(SS/TM)-GFP-LOVpep
    Use
    Tags
    Expression
    Yeast
    Mutation
    K533∆, high-affinity epitope
    Promoter
    GAL1
    Available Since
    March 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    34966-plasmid-map-sequence-id-42500

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  17. MTRAP-bio

    Plasmid
    #47746
    Purpose
    Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MTRAP
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47746-plasmid-map-sequence-id-68760

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  18. EBA140-bio

    Plasmid
    #47742
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA140
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47742-plasmid-map-sequence-id-68750

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  19. pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS

    Plasmid
    #123200
    Purpose
    binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123200-plasmid-map-sequence-id-242251

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Showing: 2161 - 2180 of 166818 results