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Showing: 2161 - 2180 of 160219 results

  5. pShuttle-CMV

    Plasmid
    #16403
    Purpose
    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only
    16403-plasmid-map-sequence-id-174093

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  6. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100902-plasmid-map-sequence-id-195323

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  7. pCI_FLAG-GFP(Y39TAG)-6His

    Plasmid
    #223507
    Purpose
    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39
    Depositor
    Edward Lemke
    Insert
    GFP(Y39TAG)
    Use
    Tags
    6xHis and FLAG
    Expression
    Mammalian
    Mutation
    Y39TAG
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223507-plasmid-map-sequence-id-447435

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  8. pR26

    Plasmid
    #127372
    Purpose
    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    tight TRE promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127372-plasmid-map-sequence-id-247988

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  10. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Article
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110770-plasmid-map-sequence-id-211461

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  12. pHN922

    Plasmid
    #178975
    Purpose
    Expression of GST-His8-GFP-nanobody
    Depositor
    Kazuhiro Abe
    Insert
    GST-His8-GFP-nanobody
    Use
    Affinity Reagent/ Antibody
    Tags
    GST, His8
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178975-plasmid-map-sequence-id-354968

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  13. pAAV-hSynapsin1-axon-GCaMP6s (AAV1)

    Viral Prep
    #111262-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-GCaMP6s (#111262). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.
    Promoter
    Syn
    Tags
    None
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only

  14. pSLQ10844

    Plasmid
    #183956
    Purpose
    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183956-plasmid-map-sequence-id-363081

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  16. pBAD-bARGSer-AxeTxe

    Plasmid
    #192473
    Purpose
    Second-generation bacterial acoustic reporter gene derived from Serratia
    Depositor
    Mikhail Shapiro
    Article
    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Inserts
    bARGSer
    AxeTxe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    the gene Ser39006_001280 was deleted from the clu…
    Promoter
    Native promoter from Enterococcus faecium and pBAD
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    192473-plasmid-map-sequence-id-376957

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  17. ZJOM65

    Plasmid
    #133654
    Purpose
    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133654-plasmid-map-sequence-id-267529

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  18. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  20. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

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Showing: 2161 - 2180 of 160219 results