We narrowed to 4,855 results for: U6...

1. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Expression

   Mutation

   Promoter

   FCP and U6

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-SpCas9-NG

   Plasmid

   #171370

   Purpose

   SpCas9-NG-mediated genome editing.

   Depositor

   Masahito Ikawa

   Article

   Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA; CBh for Cas9-2A-puroR

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAS12342_PEAR-GFP_2in1

   Plasmid

   #177184

   Purpose

   PEAR-GFP plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. B52_puro_empty_gRNA

   Plasmid

   #197557

   Purpose

   sites for cloning two gRNA with puromycin cassette

   Depositor

   Darius Bägli

   Type

   Empty backbone

   Use

   Empty grna backbone with puromycin resistance gene

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for gRNA, CMV for puromycin resistance.

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT9658-sgRNA-mCherry

   Plasmid

   #162987

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-sgMUC1_dual-MTS-3x-Set I

   Plasmid

   #162763

   Purpose

   Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiU6-hMYOD1 gRNA_1-MS2-Puro

   Plasmid

   #192673

   Purpose

   Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNA #1 (MYOD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PU6::klp-12_sgRNA

    Plasmid

    #46170

    Purpose

    klp-12 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    klp-12 targeting sgRNA (klp-12 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMA-SpCas9-g7

    Plasmid

    #80790

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. shLuc pRRL

    Plasmid

    #19125

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUB-Cas9-@GL1

    Plasmid

    #86779

    Purpose

    Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    sgRNA against GLABROUS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6-26 promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG3H-U6SC

    Plasmid

    #134755

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-35SC

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti‐puro‐OgRNA_mdxE53

    Plasmid

    #187054

    Purpose

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only