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Plasmid#185594PurposeControl lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only
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pLenti UBC hNLRP3-mNG
Plasmid#218642PurposeExpresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selectionDepositorInsertNLRP3 (NLRP3 Human)
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1
Plasmid#194936PurposeLV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD (WT)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pG1-NMGR526
Plasmid#1122DepositorUseTagsGR526ExpressionYeastMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pG1-NMGR
Plasmid#1274DepositorUseTagsGRExpressionYeastMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OMKSL-PP
Plasmid#102899PurposeEBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LMP FFB
Plasmid#89491PurposeFirefly hairpinDepositorInsertFirefly luciferase hairpin, clone B
UseMouse Targeting and RetroviralTagsExpressionMammalianMutationPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-puro
Plasmid#65232Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-neo
Plasmid#65233Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSA351_SCP1_intron_eGFP
Plasmid#206906PurposeLentiviral eGFP reporter vector with SCP1 promoter.DepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterSCP1Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PECAM-eGFP-Hygro
Plasmid#14686DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsEGFP and PGK-HygroExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRUSH
Plasmid#54479PurposeMouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sitesDepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and RNAiTagsSA-EGFP-polyA, U6 promoter, loxP, and pgk-neoExpressionMammalianMutationPromoterU6Available sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pG1-NMGR526(d22-69)
Plasmid#1125DepositorUseTagsGR526ExpressionYeastMutationnucleotides encoding NM domain amino acids 22-69 …PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
miR-144 Trap
Plasmid#60934Purposeto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoterDepositorInsertmiR-144 Trap
UseLentiviralTagsGFPExpressionMutationPromoterbidirectional phosphoglycerate kinase promoter (b…Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
miR-451 Trap
Plasmid#61487Purposeto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoterDepositorInsertmiR-451 Trap
UseLentiviralTagsGFPExpressionMammalianMutationPromoterbidirectional phosphoglycerate kinase promoter (b…Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-tTR-KRAB
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
PL552
Plasmid#68407PurposeDonor empty vector with floxed puromycin resistance gene expression cassetteDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNeoXTR f0
Plasmid#69157Purposemouse targeting vectorDepositorInsertsDTA
NeoR
GFP
AmpR
UseMouse TargetingTagsExpressionMutationPromoterAmp promoter, PGK, and noneAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PECAM-H2B-GFP-Hygro
Plasmid#14684DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsH2B-GFP and PGK-HygroExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1-Nbox-mutant
Plasmid#194937PurposeLV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-rtTR-KRAB-2SM2
Plasmid#11652PurposeTet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GST-EGFP-GPIDAF
Plasmid#213716PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
UseTagsGSTExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GST-EGFP-GPIBY55
Plasmid#213717PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 driven by an hPGK promoter.DepositorInsertEGFP
UseTagsGSTExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-GST-EGFP-GPICEAM7
Plasmid#213718PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an hPGK promoter.DepositorInsertEGFP
UseTagsGSTExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV p2GM AMPK alpha2hp1 alpha1hp1
Plasmid#89492PurposeshRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expressionDepositorInsert2 hairpins against mouse/human AMPKalpha1 and alpha2
UseRetroviralTagsExpressionMammalianMutationPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIDAF
Plasmid#213708PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIBY55
Plasmid#213707PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
N1199 launching
Plasmid#160386PurposeExpression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequenceDepositorInsertN1199
UseTagsExpressionYeastMutationPromoterPGK1Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Smad4-1719
Plasmid#49383PurposeInsert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.DepositorInsertSmad family member 4 (SMAD4 Mouse, Human)
UseLuciferaseTagsExpressionMutationPromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Smad5-1719
Plasmid#49382PurposeInsert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.DepositorInsertSmad5 (Smad5 Mouse, Human)
UseLuciferaseTagsExpressionMutationPromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.GFP
Plasmid#59404Purposeexpresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsert400 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpFOXA2Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPEasy9R
Plasmid#218291PurposePlasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.DepositorInsertpURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
natMX-ins7
Plasmid#195044PurposepFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertNatR
UseYeast genomic targetingTagstCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE reporter backbone
Plasmid#158678PurposeThis plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberryDepositorTypeEmpty backboneUseLentiviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-EGFP
Plasmid#107721PurposeFor expression of sgRNA from the U6 promoter with EGFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Geminin-mStrawberry reporter
Plasmid#158690PurposeThis plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Tnf
Plasmid#207127PurposeLuciferase vector containing 3'UTR for mouse TnfDepositorInsertTumor necrosis factor (Tnf) 3'UTR (Tnf Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-BFP
Plasmid#107722PurposeFor expression of sgRNA from the U6 promoter with BFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
LMP AMPK alpha1 hp1
Plasmid#89489PurposeshRNA against AMPK alpha1 in mouse/human to knock down protein expressionDepositorUseMouse Targeting and RetroviralTagsExpressionMammalianMutationPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only