Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 659 results for: pgk promoter

Clear keywords
Showing: 401 - 440 of 659 results

  10. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

    Enlarge

  11. psd44-Lactadherin46

    Plasmid
    #46830
    Purpose
    expression of human lactadherin in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    MFGE8 (MFGE8 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46830-plasmid-map-sequence-id-410396

    Enlarge

  12. psd44-G13WT

    Plasmid
    #46829
    Purpose
    expression of wild type G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. psd44-G13GA

    Plasmid
    #46827
    Purpose
    expression of mutant G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    G225A
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pExT

    Plasmid
    #36889
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    Expression
    Mammalian
    Mutation
    deleted nucleotides before nucleotide 275 and ins…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available Since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    36889-plasmid-map-sequence-id-46194

    Enlarge

  17. pExG

    Plasmid
    #36888
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available Since
    Aug. 17, 2012
    Availability
    Academic Institutions and Nonprofits only
    36888-plasmid-map-sequence-id-46193

    Enlarge

  18. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46826-plasmid-map-sequence-id-246440

    Enlarge

  19. psd44-G13QL

    Plasmid
    #46828
    Purpose
    expression of mutant G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Q226L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46828-plasmid-map-sequence-id-137240

    Enlarge

  22. pMT957

    Plasmid
    #228293
    Purpose
    Constitutive mutant PhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    ScPGK1 promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228293-plasmid-map-sequence-id-457079

    Enlarge

  30. LMP FFB

    Plasmid
    #89491
    Purpose
    Firefly hairpin
    Depositor
    Russell Jones
    Article
    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi
    Insert
    Firefly luciferase hairpin, clone B
    Use
    Mouse Targeting and Retroviral
    Expression
    Mammalian
    Promoter
    U6 (The GFP-Puro is from a PGK promoter; the shRN…
    Available Since
    May 25, 2017
    Availability
    Academic Institutions and Nonprofits only

  32. pMSCV-RUNX1-4A

    Plasmid
    #238534
    Purpose
    Used for overexpression of human RUNX1 in mammalian cells. Contains mutations that prevent phosphorylation at some sites of the translated protein.
    Depositor
    Diane Krause
    Article
    Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do
    Inserts
    RUNX1 (RUNX1 Human)
    Puromycin
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S249A, S266A, T273A, S276A
    Promoter
    MSCV and PGK
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    238534-plasmid-map-sequence-id-476287

    Enlarge

  37. pRUSH

    Plasmid
    #54479
    Purpose
    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites
    Depositor
    Laurie Jackson-Grusby
    Article
    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and RNAi
    Tags
    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. miR-144 Trap

    Plasmid
    #60934
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    miR-144 Trap
    Use
    Lentiviral
    Tags
    GFP
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. miR-451 Trap

    Plasmid
    #61487
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    miR-451 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 401 - 440 of 659 results