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His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

His6 and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-SpeI

Plasmid

#87331

Purpose

Expression of shRNA targeting at Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mGreenLantern

Plasmid

#195988

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR5'-attP (pCM254)

Plasmid

#68311

Purpose

Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

attP

Use

Gateway entry vector

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-iRFP_L5-L4

Plasmid

#113866

Purpose

Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
iRFP713

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I

Plasmid

#179685

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR

Expression

Mammalian

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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GST-Keima-cb5

Plasmid

#137755

Purpose

Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

Keima-cb5

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 Chfr (1014)

Plasmid

#61853

Purpose

mammalian expresion of Chfr fused to EGFP

Depositor

Jonathon Pines

Article

Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

Insert

Chfr (CHFR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
pENTR223-BcLF1

Plasmid

#198581

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcLF1

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET14-GFP30_Encap

Plasmid

#86406

Purpose

Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 30aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-mCherry

Plasmid

#79509

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

mCherry RFP

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(V266E)

Plasmid

#90089

Purpose

Expresses caspase 3 V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

V266E

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-BOLF1

Plasmid

#198579

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BOLF1

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F4

Plasmid

#37966

Depositor

Jacqueline Lees

Article

Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

Insert

E2F4 (E2F4 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Tags

TREER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TOMM20-PuRRRE

Plasmid

#208329

Purpose

PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TOMM20-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET14-GFP15_Encap

Plasmid

#86407

Purpose

Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 15aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-GAR1

Plasmid

#126873

Purpose

Expresses 3xFlag GAR1

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

GAR1 (GAR1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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