We narrowed to 6,625 results for: PROC

1. PB-hSyn-mSyp-RT1-24xGCN4

   Plasmid

   #106300

   Purpose

   The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

   Depositor

   James Zhe Liu

   Article

   Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

   Insert

   mSyp-RT1-24xGCN4 (Syp Mouse)
   

   Use

   Tags

   24xGCN4

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin1

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMKO shRNA Bad

   Plasmid

   #17237

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bad shRNA (BAD Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

3. nuc-yHS1-M7A

   Plasmid

   #159167

   Purpose

   Nuclear labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   nuc-yHS1-M7A
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSLIK-Neo-TmiR-Luc

   Plasmid

   #25743

   Purpose

   Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdTrack: shRev-Erbalpha

   Plasmid

   #22749

   Depositor

   Bruce Spiegelman

   Article

   Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

   Insert

   shRev-Erbalpha (Nr1d1 Mouse)
   

   Use

   Adenoviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pJO427

   Plasmid

   #111139

   Purpose

   URT-type vector for Glu-mIκBα expression in Mammalian cells

   Depositor

   Alexander Varshavsky

   Article

   Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

   Plasmid

   #13617

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 65, Thr 69 and Ser70 to Asp

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pTSlb-Q758C

   Plasmid

   #60729

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. [UBC][EIF4e][GG cloning]

   Plasmid

   #97189

   Purpose

   EIF4e plasmid with Pum cloning site

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Insert

   EIF4E variant 1 (EIF4E Human)
   

   Use

   Synthetic Biology

   Tags

   empty Pum cloning site

   Expression

   Mammalian

   Mutation

   Promoter

   UBC (ubiquitin C)

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR.sgKras.9

    Plasmid

    #91894

    Purpose

    sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

    Depositor

    Wen Xue

    Article

    Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Kras sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAP4K3-GFP K45E

    Plasmid

    #21734

    Depositor

    L. Miguel Martins

    Article

    Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

    Insert

    MAP4K3 (MAP4K3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of the ATP-binding site (K45E). This ver…

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

    Plasmid

    #197965

    Purpose

    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-nCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 and pUBQ10

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-24p3/LCN2-282kB mut-LUC

    Plasmid

    #46867

    Purpose

    murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    NF- kB site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pMXs-hu-c-Myc-dN2

    Plasmid

    #50774

    Purpose

    retroviral expression of human c-Myc dN2 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc -dN2 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 42-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-hu-c-Myc-dN1

    Plasmid

    #50773

    Purpose

    retroviral expression of human c-Myc dN1 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc -dN1 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 14-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-24p3/LCN2-282C/EBP mut-LUC

    Plasmid

    #46868

    Purpose

    murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    CEBP site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDN-G1Tt

    Plasmid

    #44509

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

    Plasmid

    #13616

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65, Thr 69 and Ser 70 to Alanine

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pJO428

    Plasmid

    #111141

    Purpose

    URT-type vector for Val-mIκBα expression in Mammalian cells

    Depositor

    Alexander Varshavsky

    Article

    Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET11-PC-H6

    Plasmid

    #183777

    Purpose

    Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (Pc Fly)
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. HOXB7 (human) HIS-tag pET

    Plasmid

    #8538

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pUHG10.3-TetO-Rs1

    Plasmid

    #16313

    Depositor

    Bruce Conklin

    Article

    Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

    Insert

    h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Added FLAG tag; Changed Aspartic Acid 100 to Alan…

    Promoter

    Available since

    Jan. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDN-T1GZmbh

    Plasmid

    #44522

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMKO shRNA Bmf

    Plasmid

    #17236

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf shRNA (BMF Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pBABE-hygro NM23 H1 His

    Plasmid

    #11365

    Depositor

    Ronald Kahn

    Article

    Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

    Insert

    NM23 H1 (NME1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    His118 mutation to Phe (abolishes histidine kinas…

    Promoter

    Available since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pBV-Luc/Del-7

    Plasmid

    #14968

    Depositor

    Joan Massague

    Article

    Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

    Insert

    c-myc promoter (-26/+334) (MYC Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -26/+334 (relative to the P2 transcription initia…

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pET28_6xHis-PARP1-A762V

    Plasmid

    #174795

    Purpose

    Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A762V (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A762V

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2 Notch1 LNG-6MT

    Plasmid

    #41738

    Depositor

    Raphael Kopan, Jeffrey Nye

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only