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We narrowed to 11,308 results for: ENA

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Showing: 5451 - 5500 of 11308 results

  6. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    flk1
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  10. LSB-hsa-miR-138-5p

    Plasmid
    #103233
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-138-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103233-plasmid-map-sequence-id-195515

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  14. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Article
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6ƗHis Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  15. LSB-hsa-miR-19b-3p

    Plasmid
    #103327
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-19b-3p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103327-plasmid-map-sequence-id-195703

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  16. pSC4

    Plasmid
    #91182
    Purpose
    T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    gRNAs targeting 58kb region in medicago truncatula
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91182-plasmid-map-sequence-id-182722

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  23. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid
    #124541
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    Expression
    Mammalian
    Mutation
    WT, WT
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124541-plasmid-map-sequence-id-242326

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  30. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124539
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55A, d11-41
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124539-plasmid-map-sequence-id-242319

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  33. LSB-hsa-miR-133a-3p

    Plasmid
    #103221
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-133a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-133a-3p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103221-plasmid-map-sequence-id-195491

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  34. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pChlr-4SYR

    Plasmid
    #52158
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R inā€¦
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pChlr-5SR

    Plasmid
    #52162
    Purpose
    Encodes module 5 with amino acids 12S-16R for recognition of nt 4C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16R in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pChlr-6SE

    Plasmid
    #52164
    Purpose
    Encodes module 6 with amino acids 12S-16E for recognition of nt 3G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. LSB-hsa-miR-26a-5p

    Plasmid
    #103379
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-26a-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    103379-plasmid-map-sequence-id-195807

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  42. LSB-hsa-miR-125b-5p

    Plasmid
    #103191
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-125b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-125b-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103191-plasmid-map-sequence-id-237629

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  43. pSC2

    Plasmid
    #91180
    Purpose
    T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    gRNAs targeting NCR genes
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91180-plasmid-map-sequence-id-182720

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  45. LSB-hsa-miR-101-5p

    Plasmid
    #103166
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-101-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-101-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103166-plasmid-map-sequence-id-237570

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  46. LSB-hsa-miR-7-1-3p

    Plasmid
    #103722
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-7-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-7-1-3p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103722-plasmid-map-sequence-id-196492

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  47. pChlr-4SE

    Plasmid
    #52152
    Purpose
    Encodes module 4 with amino acids 12S-16E for recognition of nt 5G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 4
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  48. pChlr-8SR

    Plasmid
    #52174
    Purpose
    Encodes module 8 with amino acids 12S-16R for recognition of nt 1C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 8
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. pChlr-7CQ

    Plasmid
    #52167
    Purpose
    Encodes module 7 with amino acids 12C-16Q for recognition of nt 2A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12C and 16E to 16Q in Module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. pChlr-7NQ

    Plasmid
    #52169
    Purpose
    Encodes module 7 with amino acids 12N-16Q for recognition of nt 2U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N and 16E to 16Q in module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5451 - 5500 of 11308 results