Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,293 results for: GFP expression plasmids

Clear keywords
Showing: 5551 - 5600 of 7293 results

  11. pCMV-T7-ABEmax-SpRY

    Plasmid
    #195279
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195279-plasmid-map-sequence-id-394869

    Enlarge

  12. pCMV-T7-ABEmax-SpG

    Plasmid
    #195278
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195278-plasmid-map-sequence-id-394868

    Enlarge

  13. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

    Enlarge

  22. TP1374

    Plasmid
    #252365
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody D9
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252365-plasmid-map-sequence-id-504601

    Enlarge

  23. TP1378

    Plasmid
    #252366
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody D6
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252366-plasmid-map-sequence-id-504537

    Enlarge

  24. TP1376

    Plasmid
    #252368
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody G9
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252368-plasmid-map-sequence-id-504517

    Enlarge

  25. TP1377

    Plasmid
    #252369
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody C10
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252369-plasmid-map-sequence-id-504764

    Enlarge

  26. TP1420

    Plasmid
    #252370
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody I
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252370-plasmid-map-sequence-id-504535

    Enlarge

  27. TP1421

    Plasmid
    #252371
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody II
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252371-plasmid-map-sequence-id-504533

    Enlarge

  28. TP1425

    Plasmid
    #252372
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody VI
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252372-plasmid-map-sequence-id-504766

    Enlarge

  29. TP1428

    Plasmid
    #252373
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody IX
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252373-plasmid-map-sequence-id-504765

    Enlarge

  30. TP1429

    Plasmid
    #252374
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody X
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252374-plasmid-map-sequence-id-504520

    Enlarge

  31. TP1431

    Plasmid
    #252375
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC Nanobody XII
    Tags
    ALFA-GFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available Since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252375-plasmid-map-sequence-id-504550

    Enlarge

  37. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid
    #163080
    Purpose
    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Inactive Mpro mutant C145A and H2B-mIFP
    Expression
    Mammalian
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163080-plasmid-map-sequence-id-320827

    Enlarge

  38. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

    Enlarge

  45. pcDNA3 H2B-mIFP T2A Mpro (wt)

    Plasmid
    #163079
    Purpose
    Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Wild type Mpro of SARS-CoV-2 and H2B-mIFP
    Expression
    Mammalian
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163079-plasmid-map-sequence-id-320826

    Enlarge

  48. pJUMP19-Tac

    Plasmid
    #126958
    Purpose
    Terminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI.
    Depositor
    Chris French
    Article
    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126958-plasmid-map-sequence-id-248014

    Enlarge

  50. AD59_pEP.DonorCLYBL.TS

    Plasmid
    #199227
    Purpose
    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199227-plasmid-map-sequence-id-391392

    Enlarge

Showing: 5551 - 5600 of 7293 results