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Plasmid#202138PurposeLevel 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructsDepositorInsertDual 2A peptide
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWB511-TP-BVRA
Plasmid#203480PurposeFor Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.DepositorInsertRattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Silene_conica _GlnRS_MitoTP_pK7FWG2
Plasmid#202654PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _LysRS_MitoTP_pK7FWG2
Plasmid#202655PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Agrostemma_githago _LysRS_MitoTP_pK7FWG2
Plasmid#202656PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _TyrRS_MitoTP_pK7FWG2
Plasmid#202657PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_vulgaris _TyrRS_MitoTP_pK7FWG2
Plasmid#202658PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _PheRS_MitoTP_pK7FWG2
Plasmid#202659PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _PheRS_PlastidTP_pK7FWG2
Plasmid#202660PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2
Plasmid#202661PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_CytC_TP_GFP_pK7FWG2
Plasmid#202646PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative Cytochrome C
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
1BR9-AtWUS
Plasmid#193865PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.DepositorInsertAtWUS E. Coli Codon Optimized
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_CydAB-mNeongreen
Plasmid#182393PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusionsDepositorInsertsVNp-LZ
CydAB-mNeongreen
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptide and SV40 NLSExpressionBacterialMutationPromoterD-mannoseAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056H
Plasmid#183135PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056J
Plasmid#183136PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056K
Plasmid#183137PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MUM2SP-Citrine
Plasmid#181963PurposeMUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertMUM2 signal peptide, fused to the Citrine YFP in pAD
UseTagsCitrine YFPExpressionPlantMutationPromoterMUM4pro 1.5kb fragmentAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-14
Plasmid#160714PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDS12-MpER-Targ
Plasmid#136094PurposeCDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'endDepositorInsertER targeting peptide from predicted chitinase Mapoly0069s0092
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits