We narrowed to 4,269 results for: Lta

1. pBSWHhc-Lam

   Plasmid

   #133902

   Purpose

   Negative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   LMNA (Lam) (LMNA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223321

   Purpose

   It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   SpCas9 codon optimized for human (cas9 Human, Synthetic)
   chloramphenicol resistance protein
   CcdB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB2076 pAAV REPAIR.t1

   Plasmid

   #176323

   Purpose

   pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Inserts

   human codon optimized Cas13bt1 (catalytically inactivated)
   huADAR2dd(E488Q) (ADARB1 Human)
   Cas13bt1 crRNA + BpiI cloning site
   

   Use

   AAV and CRISPR

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   E488Q, only the deaminase domain (aa 276-702 are …

   Promoter

   EFS (short EF1alpha) and hU6

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MLRV2: NF-kb-SMAD-DBE-P53-AP1

   Plasmid

   #124536

   Purpose

   Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Inserts

   5 copies of the NF-kb DNA binding motif
   7xSMAD_RE::FLuc
   3xDBE_BE::Renilla (FOXO)
   2xP53_RE::NLuc
   6xAP-1_RE::GrRenilla
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

   Plasmid

   #215769

   Purpose

   encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

   Depositor

   Dimitrios Wagner

   Article

   Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

   Inserts

   TRAC-5' homology arm (TRAC Human)
   P2A-CD19.28.zeta-bgH (CD19 Human, Mouse, Bovine)
   TRAC-3' homology arm (TRAC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cas12a PAM mutated

   Promoter

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

   Plasmid

   #203797

   Purpose

   Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Article

   Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

   Inserts

   PIK3CA (PIK3CA Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-DIO-Magneto2.0-sNRPpA

   Plasmid

   #74307

   Purpose

   Cre-dependent expression of Magneto2.0 in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
   

   Use

   AAV and Cre/Lox

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TRPV4: delta 760-871

   Promoter

   CMV (after Cre recombination)

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

   Plasmid

   #203798

   Purpose

   Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Article

   Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

   Inserts

   PIK3CA (PIK3CA Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H1047R

   Promoter

   Available since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

   Plasmid

   #115890

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]

    Plasmid

    #115893

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Has Service

    AAV8

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

    Plasmid

    #115891

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α promoter (1.1kb short version)

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid

    #231235

    Purpose

    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TAD deleted (delta1-143), DBD deleted (delta340-4…

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23-hs737.1

    Plasmid

    #222473

    Purpose

    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 1). Variant 1 is chr10:128568698-A-G where the information is chromosome:positionInB38-RefAllele-AltAllele.

    Depositor

    Tychele Turner

    Article

    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

    Insert

    hs737 enhancer sequence (sequence containing variant 1) (LOC110120928 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Variant 1 is chr10:128568698-A-G where the inform…

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23-hs737.3

    Plasmid

    #222475

    Purpose

    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 3). Variant 3 is chr10:128569437-G-A where the information is chromosome:positionInB38-RefAllele-AltAllele.

    Depositor

    Tychele Turner

    Article

    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

    Insert

    hs737 enhancer sequence (sequence containing variant 3) (LOC110120928 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Variant 3 is chr10:128569437-G-A where the inform…

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23-hs737.2

    Plasmid

    #222474

    Purpose

    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 2). Variant 2 is chr10:128569418-T-C where the information is chromosome:positionInB38-RefAllele-AltAllele.

    Depositor

    Tychele Turner

    Article

    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

    Insert

    hs737 enhancer sequence (sequence containing variant 2) (LOC110120928 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Variant 2 is chr10:128569418-T-C where the inform…

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

    Plasmid

    #187292

    Purpose

    Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    AH-deltaC2, RhoA Q63L

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid

    #105842

    Purpose

    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (AAV8)

    Viral Prep

    #60658-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (#60658). In addition to the viral particles, you will also receive purified pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato plasmid DNA. CAG-driven, Flp-dependent expression of the voltage-gated Na+ channel mNaChBac-T2A-tdTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Promoter

    EF1α

    Tags

    None

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

19. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit

    #1000000156

    Purpose

    The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.

    Depositor

    Scott Lenaghan

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

20. 4G-Cloning Plasmid Kit

    Plasmid Kit

    #1000000252

    Purpose

    The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.

    Depositor

    Stephan Gruber

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

21. Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)

    Plasmid Kit

    #1000000175

    Purpose

    Plasmids and entry vectors for diverse fluorescent sensors of physiological parameters in cells.

    Depositor

    Adam Cohen

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

22. pH-STEME-1-esgRNA

    Plasmid

    #138135

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pH-STEME-NG-esgRNA

    Plasmid

    #138136

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. STEME-1

    Plasmid

    #138137

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMJA251

    Plasmid

    #128540

    Purpose

    Acceptor vector for gamma and Delta TCR

    Depositor

    Marcos Alcocer

    Insert

    truncated human TCR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. STEME-4

    Plasmid

    #138140

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    wtTadA-TadA7.10-APOBEC3A-nCas9-2X(T2A-UGI-NLS)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. STEME-3

    Plasmid

    #138139

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. STEME-2

    Plasmid

    #138138

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    wtTadA-TadA7.10-APOBEC3A-nCas9-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XE238 hVangl2/Strabismus pCS2P+

    Plasmid

    #16992

    Depositor

    Randall Moon

    Insert

    Vangl2/Strabismus (VANGL2 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

30. LV-tetO-EF1a-GFP

    Plasmid

    #187645

    Purpose

    Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites

    Depositor

    Martin Carroll

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge