We narrowed to 19,628 results for: REN;

1. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

   Plasmid

   #45578

   Purpose

   Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a-AlkB deltaN11

   Plasmid

   #73622

   Purpose

   Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

   Depositor

   Catherine Drennan

   Article

   Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   AlkB (alkB E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deletion of first 11 AA

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pMH-HT_SsCHAD

   Plasmid

   #127783

   Purpose

   Expresses SsCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ALK7 wt

   Plasmid

   #80885

   Purpose

   mammalian expression of ALK7 WT

   Depositor

   Aristidis Moustakas

   Article

   Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

   Insert

   ALK7 (Acvr1c Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pJUMP18-pheS_NOC

   Plasmid

   #127031

   Purpose

   Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part PheS_NOC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   pheS A294G promiscuous mutation

   Promoter

   Available since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJUMP27ts[mCherry]-1A(lacZ)

   Plasmid

   #126980

   Purpose

   Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   RepA A56V thermosensitive mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MyoD-cTAP

   Plasmid

   #20915

   Depositor

   Stephen Tapscott

   Article

   Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

   Insert

   MyoD cTAP (Myod1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   TAP tag is fused to the 3' end of the MyoD s…

   Promoter

   Available since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

8. HA PRAS40 S183A

   Plasmid

   #15674

   Depositor

   John Lawrence

   Article

   Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

   Insert

   PRAS40 S183A (AKT1S1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S183A

   Promoter

   Available since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

9. IRE-nonOpt-TREAT

   Plasmid

   #196922

   Purpose

   The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   non-optimal Renilla luciferase
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA PRAS40 F129A

    Plasmid

    #15673

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 F129A (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F129A

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pAAV.Syn-FLEX.iGABASnFR.F102Y.Y137L.mRuby3

    Plasmid

    #112171

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Tags

    mRuby3

    Expression

    Mutation

    F102Y.Y137L

    Promoter

    Synapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5 KSR1 S392A

    Plasmid

    #25976

    Depositor

    Rob Lewis

    Article

    Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed serine 392 to alanine

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCVL SFFV Sbf-Sal linker

    Plasmid

    #50434

    Purpose

    Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-STOP-shA1

    Plasmid

    #14422

    Depositor

    Klaus Rajewsky

    Article

    Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

    Insert

    modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
    

    Use

    Cre/Lox and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJUMP18-B0034-MV_RN

    Plasmid

    #127017

    Purpose

    Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part B0034m-MV_RN
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS cErm (CT#1054)

    Plasmid

    #13907

    Depositor

    Cliff Tabin

    Article

    Brent et al Development. 2004 Aug . 131(16):3885-96.

    Insert

    Erm (ETV5 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET29NK-LGK/mGFPmut3

    Plasmid

    #81168

    Purpose

    GFP fusion to levoglucosan kinase

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

    Insert

    Levoglucosan kinase wild-type
    

    Use

    Tags

    His6x and mGFPmut3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TOPO CMV

    Plasmid

    #68454

    Purpose

    TOPO construct expressing CMV for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cytomegalovirus Immediate-Early Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. hPlekhm1_pIRES puro Glue

    Plasmid

    #73453

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5 KSR1 T274V

    Plasmid

    #25975

    Depositor

    Rob Lewis

    Article

    Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed threonine 274 to valine

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only