Skip to main content
Addgene

We narrowed to 11,219 results for: NSI

Showing: 4721 - 4760 of 11219 results
  1. pYPQAT37

    Plasmid
    #223409
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.
    Depositor
    Insert
    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pYPQAT38

    Plasmid
    #223410
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.
    Depositor
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pYPQAT39

    Plasmid
    #223411
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.
    Depositor
    Insert
    2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pYPQAT40

    Plasmid
    #223412
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.
    Depositor
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pYPQAT41

    Plasmid
    #223413
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.
    Depositor
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pYPQAT42

    Plasmid
    #223414
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pYPQAT43

    Plasmid
    #223415
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Insert
    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR; T-dna vector
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pYPQAT06

    Plasmid
    #223378
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.
    Depositor
    Insert
    ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Use
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pJUB2x

    Plasmid
    #208814
    Purpose
    Encodes GFP under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell
    Depositor
    Insert
    Two copies of JUB1 binding site within the synthetic promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    JUB1 TF-responsive promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pJB53

    Plasmid
    #226309
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4721 - 4760 of 11219 results