We narrowed to 11,219 results for: NSI
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Plasmid#223409PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT38
Plasmid#223410PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT39
Plasmid#223411PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.DepositorInsert2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT40
Plasmid#223412PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT41
Plasmid#223413PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT42
Plasmid#223414PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT43
Plasmid#223415PurposeT-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.DepositorInsertZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
UseCRISPR; T-dna vectorTagsNLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT06
Plasmid#223378PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
cASIC1 concatemer - fully assembled
Plasmid#233133PurposeTo create cASIC1 channels of defined stoichiometry in mammalian cellsDepositorInsertASIC1 (ASIC1 Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
cASIC1 concatemer - Subunit C
Plasmid#233131PurposeTo create cASIC1 channels of defined stoichiometry in mammalian cellsDepositorInsertASIC1 (ASIC1 Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
cASIC1 concatemer - Subunit B
Plasmid#233130PurposeTo create cASIC1 channels of defined stoichiometry in mammalian cellsDepositorInsertASIC1 (ASIC1 Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
cASIC1 concatemer - Subunit A
Plasmid#233129PurposeTo create cASIC1 channels of defined stoichiometry in mammalian cellsDepositorInsertASIC1 (ASIC1 Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
hnRNPAB X2-FL
Plasmid#216515PurposeExpresses MBP-tagged full length hnRNPAB X2DepositorInserthnRNPAB X2 FL
UseTags6xHis tags-MBPExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SUMO-LS4
Plasmid#228450PurposeLS4 protein expression vector for bacteriaDepositorInsertLS4
UseTags10xHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SUMO-LS12
Plasmid#228451PurposeLS12 protein expression vector for bacteriaDepositorInsertLS12
UseTags10xHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-SP1
Plasmid#232649Purposeexpresses SP1 in mammalian cellsDepositorInsertSP1 (SP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif1A-EGFP-SNAP
Plasmid#229851PurposeKif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif1A-EGFP-SNAP (Kif1a Rat)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_YafQ/DinJ
Plasmid#229148PurposeToxin-Antitoxin (Dinj/YafQ)DepositorInsertmCherry, DinJ/YafQ
UseTagsExpressionBacterialMutationPromoterPtlpA (mCherry), DinJ/YafQ (Native)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_Txe/Axe
Plasmid#229147PurposeToxin-Antitoxin (Txe/Axe)DepositorInsertmCherry, Txe/Axe
UseTagsExpressionBacterialMutationPromoterPtlpA (mCherry), Txe/Axe (Native)Available sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianMutationPromoterEF-1-alpha promoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC1_pPromALB_enh5898_FireflyLuc
Plasmid#220285PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 5898
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC2_pPromALB_enh29774_FireflyLuc
Plasmid#220286PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 29774
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC3_pPromALB_enh50363_FireflyLuc
Plasmid#220287PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 50363
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC4_pPromALB_enh50358_FireflyLuc
Plasmid#220288PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 50358
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJUB2x
Plasmid#208814PurposeEncodes GFP under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cellDepositorInsertTwo copies of JUB1 binding site within the synthetic promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJUB1 TF-responsive promoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Talin-1 WT
Plasmid#201798PurposeExpresses Talin-1 protein in MAC-Tag-C backboneDepositorInsertTalin-1 (Tln1 Mouse)
UseTagsBirA, HA, strepIIIExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSKm4-ATF1
Plasmid#221133PurposeFormaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detectionDepositorInsertATF1 (ATF1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterPm4Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNS3_Ptrc::lasR_Plas::mNG
Plasmid#189573PurposeFluorescent reporter for Las activation by 3OC12-HSLDepositorInsertsTranscriptional activator lasR
Monomeric Neon Green
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlas and trcAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB53
Plasmid#226309PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-3
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionBacterialMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-coSnaA-TEV-His_His-TEV-SnaC
Plasmid#225059Purposerecombinant expression of SnaA in E. coliDepositorInsertSnaA
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
WNV NS2B-NS3 protease (catalytically active, self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204795PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET-Cm-CspB
Plasmid#215400PurposeCm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)DepositorInsertcspB
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA2
Plasmid#215399PurposeCm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)DepositorInsertcspA2
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-11_humanized-mutant
Plasmid#214316PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (Casp4 Mouse)
UseRetroviralTagsMycExpressionMutationKLS288YKT_KASIHS348TPRAKAPromoterMESVAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS18
Plasmid#215675PurposeCas9 + guide plasmid for inserting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS62
Plasmid#215676PurposeCas9 + guide plasmid for inserting ChrIII split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS77
Plasmid#215681PurposeCas9 + guide plasmid targeting ChrIII split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_R269D
Plasmid#214310PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationR269DPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_K356D
Plasmid#214309PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationK356DPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only