We narrowed to 18,708 results for: met

1. pTriEx-NTOM20-mVenus-Zdk3

   Plasmid

   #81012

   Purpose

   Expression of Zdk1 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk3
   

   Use

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIntegrase_5

   Plasmid

   #60577

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK408 (mAID-Nluc Hygro)

   Plasmid

   #121198

   Purpose

   C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   mAID-Nluc Hygro
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTagRFP-T-Cx43-7

   Plasmid

   #58009

   Purpose

   Localization: Gap Junctions, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Cx43
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mTagRFP-T-Lysosomes-20

   Plasmid

   #58022

   Purpose

   Localization: Lysosome Membrane, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LAMP1
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCFJ1662

   Plasmid

   #51482

   Purpose

   miniMos transposable element with HygroR, MCS cloning

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

   Plasmid

   #110623

   Purpose

   It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   PH mScarlet-I- IRES- sYFP2 PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-T3G-ERP2

   Plasmid

   #175188

   Purpose

   All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. peCALWY-NB

   Plasmid

   #22239

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY non-binding variant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C284S, C287S, C413S and C416S mutations linker c…

   Promoter

   Available Since

   Jan. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSMVP-Cas9FL-P2A-turboGFP

    Plasmid

    #80941

    Purpose

    Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9FL
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ1663

    Plasmid

    #51484

    Purpose

    miniMos transposable element with HygroR and peel-1, MCS cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas_5+7_gfp

    Plasmid

    #60595

    Purpose

    encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_5+7_gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas_2+5

    Plasmid

    #60594

    Purpose

    encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_2+5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSFDuet-1_DARPinOFF7

    Plasmid

    #182396

    Purpose

    Bacterial expression of DARPinOFF7

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DARPinOFF7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRATIO3212

    Plasmid

    #141311

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    mScarlet-I and mVenus

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRATIO3267

    Plasmid

    #141312

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    gLUC and redLUC

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ1655

    Plasmid

    #51481

    Purpose

    miniMos transposable element with HygroR, R4-R3 Gateway cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M-SP-sgRNA

    Plasmid

    #48671

    Purpose

    Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEB1-moxVenus

    Plasmid

    #103993

    Purpose

    Plasmid encoding moxVenus

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxVenus
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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