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167,318 results

Showing: 2481 - 2500 of 167318 results

  2. PB CAG-L-3xSV40pA-L-td-sfGFP

    Plasmid
    #133574
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133574-plasmid-map-sequence-id-274064

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  6. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid
    #219964
    Purpose
    Express human BCR-ABL1-P190 in mammalian cells
    Depositor
    Mohamed Fareh
    Insert
    BCR-ABL1-P190 (ABL1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    219964-plasmid-map-sequence-id-442484

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  7. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Article
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available Since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

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  8. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  12. pJA54 HLA-A*03:01

    Plasmid
    #180451
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*03:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180451-plasmid-map-sequence-id-355758

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  13. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196061-plasmid-map-sequence-id-385874

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  14. pLVX-Tet3G blasticidin

    Plasmid
    #128061
    Purpose
    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128061-plasmid-map-sequence-id-255115

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  18. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  19. pRRLsinPGK_IRFP720_WPRE

    Plasmid
    #91785
    Purpose
    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    iRFP720
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91785-plasmid-map-sequence-id-180617

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  20. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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Showing: 2481 - 2500 of 167318 results