Addgene

We narrowed to 10,134 results for: AGA

Showing: 3751 - 3780 of 10134 results

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pCold I-Hero9

Plasmid

#187927

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero9

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero9

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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gCOTS-pyl

Plasmid

#92048

Purpose

This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

Depositor

Lital Alfonta

Article

Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

Inserts

Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)

Use

Cyanobacteria s. elongatus genome recombination i…

Tags

Expression

Mutation

Promoter

LeuP (native S. elongatus promoter) and PrcbL

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ING5

Plasmid

#13290

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING5 (ING5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCAG Fzd7 GFP

Plasmid

#66106

Purpose

expression of chicken Fzd7 GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

Fzd7 (FZD7 Chicken)

Use

Chicken expression

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shABCB7_1

Plasmid

#102968

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_1 (ABCB7 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_CIC_iso1_1

Plasmid

#135743

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 3

Plasmid

#36103

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 3 (Gng3 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
spa-GCaMP6s

Plasmid

#67556

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6s

Use

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shHmga1#1

Plasmid

#122288

Purpose

Knockdown for mouse Hmga1

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga1 (Hmga1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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HsSPHK2

Plasmid

#118092

Purpose

Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL1, 10

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 219-465

Plasmid

#45972

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 219-465 (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

Plasmid

#42040

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532, aa716-772 and aa804-924, F951A…

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

Plasmid

#170183

Purpose

Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

Depositor

Hunter Fraser

Article

Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

Insert

sgBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 4

Plasmid

#36114

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B4 (Gnb4 Mouse)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Use

Tags

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 3

Plasmid

#36113

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B3 (GNB3 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pCRRNA-G20-R20

Plasmid

#101822

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shPRDM14-3

Plasmid

#193696

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MBD3L2

Plasmid

#106332

Purpose

All-in-one lentivector with tet-inducible MBD3L2

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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Empty Anchor Control

Plasmid

#127665

Purpose

Encodes for puromycin resistance expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

Puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF1b

Plasmid

#67494

Purpose

Expression of shRNA targeting human ARF1b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF1 shRNA (ARF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pTRIPZ-DoxOn-shCUX1-5150

Plasmid

#90469

Purpose

Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

Depositor

Alain Nepveu

Article

Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

Insert

TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 13

Plasmid

#36110

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 13 (GNG13 )

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pET21a(+) LdtMt2 (131-408)

Plasmid

#59337

Purpose

Expresses LdtMt2 for L,D-transpeptidation

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

LdtMt2 (131-408)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCK051 p5E exorh:mCerulean

Plasmid

#195952

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:mCerulean

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 12

Plasmid

#36109

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 12 (Gng12 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
CTA2-mNG211

Plasmid

#141167

Purpose

Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

SUMO-CTA2-mNG211

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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