We narrowed to 20,380 results for: REN;

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TAF15_RRM-ZNF_pGEX

Plasmid

#135111

Purpose

Recombinant expression of TAF15 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

TAF15 (TAF15 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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HA-VHL 1-115-pBabe-puro

Plasmid

#19243

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 116 - 213 (end)

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-CocE

Plasmid

#114600

Purpose

"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

CocE

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC12)_Psyn-sgRNArodA

Plasmid

#149661

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC12), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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FUP95PDZGW (C5)

Plasmid

#74021

Purpose

lentiviral expression of Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

PDZ

Promoter

hUbC

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
pICH-chl-roGFP2-GPXC71S

Plasmid

#191703

Purpose

Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

chl-redox(ro)GFP2-GPXC71S

Use

Binary agrobacterium ti plasmid

Expression

Plant

Mutation

Peroxidatic Cysteine 71 replaced with Serine

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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Ef1a-QCP-3xBFP

Plasmid

#158206

Purpose

Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

QCP under the Elfa promoter and three BFP tags

Use

Synthetic Biology

Tags

3xBFP

Expression

Mammalian

Promoter

Elfa

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

Plasmid

#149588

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 HA-PNRC1

Plasmid

#123294

Purpose

Mammalian expression of PNRC1 N-HA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Tags

HA

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGST-TEV-yVms1(1-417,delta(38-69))

Plasmid

#128739

Purpose

Bacterial overexpression and crystallization

Depositor

Chris Hill

Article

Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

Insert

Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

residues 1-417 with deletion of 38-69

Promoter

T7

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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gh124

Plasmid

#106794

Purpose

expression of gRNA targeting ST8SIA4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA4 (ST8SIA4 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pLTR PKC alpha

Plasmid

#8416

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
SRSF11_RRM_pGEX

Plasmid

#135121

Purpose

Recombinant expression of SRSF11 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF11 (SRSF11 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Oct. 11, 2020

Availability

Academic Institutions and Nonprofits only

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D3 clone (p21/WAF1 promoter)

Plasmid

#16452

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pGEX Aps-SH2

Plasmid

#46404

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Aps (SH2B2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 404-523

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E836K)

Plasmid

#29542

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pMTH PKC beta II

Plasmid

#8410

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Rat Protein Kinase C beta II (Prkcb Rat)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pTRCHisB-TRF2_M

Plasmid

#50490

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Myb (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 438-500

Promoter

pTrc

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pIhh742bp-Luc

Plasmid

#53605

Purpose

Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

Ihh promoter

Use

Luciferase; Reporter

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-parkin W403A-C431S

Plasmid

#45977

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A-C431S (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Doub…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

Plasmid

#149590

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro shPRDM14-1

Plasmid

#193694

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shPRDM14-2

Plasmid

#193695

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro shPRDM14-3

Plasmid

#193696

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro shPRDM14-4

Plasmid

#193697

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro shPRDM14-5

Plasmid

#193698

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-CytERM-N-17

Plasmid

#56158

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CytERM

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB416

Plasmid

#68681

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB314

Plasmid

#68642

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
A3H HapII-Cas9n-UGI

Plasmid

#119141

Purpose

Base editor made from APOBEC3H Haplotype II

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype II-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG II-BTLA-mGFP

Plasmid

#180798

Purpose

Expression of mouse BTLA fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLA (Btla Mouse)

Use

Retroviral

Tags

mEGFP

Promoter

MSCV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-ERBB4 (E542K)

Plasmid

#29539

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
MEIS1A-pET

Plasmid

#21015

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Tags

HIS and T7

Expression

Bacterial

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-fSFRP2-GFP

Plasmid

#22915

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu secreted frizzled receptor protein 2 promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-E1.1-NRSE-RFP

Plasmid

#22929

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
NKP33 GFP (1032)

Plasmid

#62022

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

C9orf46 (PLGRKT Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pDx_mScarlet-I-SYFP2

Plasmid

#189761

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJR2953

Plasmid

#122549

Purpose

Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemCardinal2

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB231

Plasmid

#68622

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syk(C)-SH2

Plasmid

#46519

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(C) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 149-263

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat4-SH2

Plasmid

#46514

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat4 (STAT4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 133-678

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA pDEST40 Cas9 GW STOP

Plasmid

#183196

Purpose

Destination vector

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
N-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45239

Purpose

sensor targeted to N-terminus of Hec1

Depositor

Michael Lampson

Article

Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

Insert

Hec1, Aurora B FRET sensor

Tags

Aurora B FRET sensor and Hec1

Expression

Mammalian

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mGold-CAF1-C-10

Plasmid

#158003

Purpose

Visualization of nucleus in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

CAF1 (Chaf1a Mouse)

Tags

mGold

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RBM45_Full-length_pGEX

Plasmid

#135127

Purpose

Recombinant expression of RBM45 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM45 (RBM45 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Ad-HisFlag-USP19CYT

Plasmid

#155245

Purpose

for generating adenoviruses expressing the cytoplasmic isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (Cytoplasmic isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB117

Plasmid

#68571

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6401 - 6450 of 20380 results

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