We narrowed to 9,523 results for: pcas

1. pCAM44

   Plasmid

   #86484

   Purpose

   Expresses cbbL-mRFP1

   Depositor

   Pamela Silver

   Article

   Myhrvold et al Nat Commun. 2015 Nov 30;6:10039. doi: 10

   Insert

   cbbL-mRFP1
   

   Tags

   mRFP1

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAM45

   Plasmid

   #86485

   Purpose

   Expresses csoS1A-mRFP1

   Depositor

   Pamela Silver

   Article

   Myhrvold et al Nat Commun. 2015 Nov 30;6:10039. doi: 10

   Insert

   csoS1A-mRFP1
   

   Tags

   mRFP1

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAM69

   Plasmid

   #86486

   Purpose

   Expresses pduA-mRFP1

   Depositor

   Pamela Silver

   Article

   Myhrvold et al Nat Commun. 2015 Nov 30;6:10039. doi: 10

   Insert

   pduA-mRFP1
   

   Tags

   mRFP1

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAM70

   Plasmid

   #86487

   Purpose

   Expresses eutM-mRFP1

   Depositor

   Pamela Silver

   Article

   Myhrvold et al Nat Commun. 2015 Nov 30;6:10039. doi: 10

   Insert

   eutM-mRFP1
   

   Tags

   mRFP1

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAM72

   Plasmid

   #86488

   Purpose

   Expresses T4_gp23-mRFP1

   Depositor

   Pamela Silver

   Article

   Myhrvold et al Nat Commun. 2015 Nov 30;6:10039. doi: 10

   Insert

   T4_gp23-mRFP1
   

   Tags

   mRFP1

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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6. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Plasmid

   #101215

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A and R1060A

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA-mTmG

   Plasmid

   #26123

   Depositor

   Liqun Luo

   Insert

   Precursor mT/mG
   

   Tags

   membrane tag

   Expression

   Mammalian

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas-CmR(+)

    Plasmid

    #113633

    Purpose

    Chloramphenicol resistant derivative of pCas plasmid

    Depositor

    Olivier Lichtarge

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    Chloramphenicol resistance gene
    

    Use

    CRISPR

    Promoter

    Pcat

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas-dCas12m

    Plasmid

    #192275

    Purpose

    Encodes MmdCas12m (D485A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas-Cas12m

    Plasmid

    #192274

    Purpose

    Encodes MmCas12m under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA-FLEX

    Plasmid

    #38041

    Depositor

    Naoshige Uchida

    Article

    Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CA

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA-ZtTA

    Plasmid

    #26083

    Depositor

    Liqun Luo

    Article

    Li et al PLoS One. 2010 . 5(7):e11503.

    Insert

    a loxP-flanked β-geo preceding the tetracycline transactivator tTA
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas-mut4_1_2_VA

    Plasmid

    #186441

    Purpose

    Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid

    #101217

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG:GPI-GFP

    Plasmid

    #32601

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Rhee et al Genesis. 2006 Apr;44(4):202-18.

    Insert

    GPI-GFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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