168,951 results

1. pGEX-4T-3-mR7BD

   Plasmid

   #79149

   Purpose

   bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

   Depositor

   Aimee Edinger

   Article

   Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

   Insert

   R7BD (Rilp Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 220-299

   Promoter

   tac

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

   Plasmid

   #64512

   Purpose

   Expression of St1 dCas9-3XTagBFP2 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmTagBFP2

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK419 (GAL-OsTIR1(F74G))

   Plasmid

   #140656

   Purpose

   GAL-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74G)
   

   Expression

   Yeast

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV9)

   Viral Prep

   #100837-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   Syn

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pDZ148

   Plasmid

   #41950

   Depositor

   Stephen Elledge

   Article

   Zhang et al Cell. 2006 Aug 11;126(3):529-42.

   Insert

   ubiquitin specific peptidase 28 (USP28 )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pORANGE GFP-Actb KI

   Plasmid

   #131479

   Purpose

   Endogenous tagging of βactin: N-terminal (amino acid position: D2)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Actb, Actb Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn1-DIO-ExRai-AKAR2

   Plasmid

   #171846

   Purpose

   Cre-dependent, synapsin promoter-driven expression of the green fluorescent ExRai-AKAR2 PKA biosensor in neurons.

   Depositor

   Richard Huganir

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLAG-6a-Ataxin3Q22

   Plasmid

   #22126

   Depositor

   Henry Paulson

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

9. 330-Plakoglobin-Myc

   Plasmid

   #32228

   Depositor

   Kathleen Green

   Article

   Palka et al J Cell Sci. 1997 Oct;110 ( Pt 19):2359-7

   Insert

   Plakoglobin (JUP Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   human beta-actin

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0056

    Plasmid

    #142529

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MAF (MAF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3

    Plasmid

    #172922

    Purpose

    For enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3

    Depositor

    GENIE Project

    Has Service

    AAV1 and AAV9

    Insert

    axon-jGCaMP8m-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain (N terminal on insert…

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid

    #207792

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pYPQ203 (pMDC32-Ubi1)

    Plasmid

    #86207

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Expression

    Plant

    Promoter

    Maize ubiquitin 1 (ZmUbi1)

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MscL(G22S)-c-term-cpGFP-ERexp

    Plasmid

    #195346

    Purpose

    Control plasmid for the MscL tension biosensor

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)-c-term-cpGFP-ERexp
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMVd2

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1_SPTLC1_WT

    Plasmid

    #181920

    Purpose

    3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

    Depositor

    Christian Metallo

    Article

    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

    Insert

    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
    

    Use

    Lentiviral; Mammalian expression, , doxycycline i…

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. beta-catenin (S33Y)-pcw107

    Plasmid

    #64615

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Mutation

    S33Y

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh92

    Plasmid

    #106763

    Purpose

    expression of gRNA targeting FUT8

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT8 (FUT8 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_VHL_WT

    Plasmid

    #81874

    Purpose

    Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    VHL (VHL Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF-MCM4/6

    Plasmid

    #116951

    Purpose

    Expresses human MCM4 and human MCM6

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM6 (MCM6 Human)
    Human MCM4 (MCM4 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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