167,318 results

1. pDule2-Mb haloTyrRS C6

   Plasmid

   #160378

   Purpose

   C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

   Depositor

   Ryan Mehl

   Article

   Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

   Insert

   C6 HaloTyrosine tRNA synthatase
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Mammalian

   Mutation

   L270S, Y271L, N311G, C313T

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. NeonGreen

   Plasmid

   #197924

   Purpose

   Monomer expression vector

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

   Insert

   NeonGreen
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCgCas9_recT

   Plasmid

   #153337

   Purpose

   Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-E-Syt1

   Plasmid

   #66833

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt1 (ESYT1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIB2

   Plasmid

   #25451

   Depositor

   Benjamin Glick

   Article

   Sears et al Yeast. 1998 Jun 15;14(8):783-90.

   Insert

   Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
   

   Use

   Pichia pastoris expression

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. IFN-Beta_pGL3

   Plasmid

   #102597

   Purpose

   IFN-beta promoter driving luciferase. Use in IFN reporter assay.

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   Human IFN-Beta promoter (IFNB1 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

   Plasmid

   #203840

   Purpose

   Kir2.1 expression for silencing neurons and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Has Service

   AAV8

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   2A-mScarlet-KASH

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-Puro-CAG-mNG2(1-10)

   Plasmid

   #206042

   Purpose

   Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
   

   Use

   CRISPR and Synthetic Biology; Donor template

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

    Viral Prep

    #112677-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

11. Flag-GFP-HRP-TM_pLX304

    Plasmid

    #202021

    Purpose

    expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-HRP-TM
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-Peredox

    Plasmid

    #73807

    Purpose

    Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

    Depositor

    Gary Yellen

    Article

    Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

    Insert

    Peredox
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-D1ER

    Plasmid

    #36325

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1ER second generation cameleon (calcium sensor) targeted to ER
    

    Tags

    KDEL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOK

    Plasmid

    #166651

    Purpose

    Suicide vector for gene manipulation

    Depositor

    Dirk Bumann

    Article

    Cianfanelli et al BMC Microbiol. 2020 May 24;20(1):129. do

    Insert

    I-sceI, sacB
    

    Use

    Synthetic Biology

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

    Plasmid

    #216489

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX311-Cas9

    Plasmid

    #118018

    Purpose

    Constitutive expression of Cas9

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Cas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3.1(+)-NEMOb

    Plasmid

    #189934

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOb
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP_sfCherry2(11)_zyxin

    Plasmid

    #83034

    Purpose

    Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_zyxin
    

    Expression

    Mammalian

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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