We narrowed to 152,655 results for: des

1. Str-Ii_SBP-mCherry-Golgin84

   Plasmid

   #65304

   Purpose

   synchronize trafficking of Golgin-84 from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pExpreS2-1-GFP

   Plasmid

   #175363

   Purpose

   ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Insert

   sgGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   F64L, S65C, and I167T

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWzl_CRKL-W275L

   Plasmid

   #196019

   Purpose

   Expresses CRKL W275L

   Depositor

   William Hahn

   Article

   Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

   Insert

   CRKL
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1-Bxb1-LP-TC

   Plasmid

   #183754

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox257
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRIP-SFFV-GFP-RELA

   Plasmid

   #196629

   Purpose

   Express GFP-RELA

   Depositor

   Nicolas Manel

   Article

   Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

   Insert

   GFP-RELA (RELA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3b-8XGliBS:EGFP

   Plasmid

   #84602

   Purpose

   Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

   Depositor

   James Chen

   Article

   Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   8X Gli-binding site

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-NurRE3

   Plasmid

   #208253

   Purpose

   pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

   Depositor

   Douglas Kojetin

   Article

   Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

   Insert

   3X Nur response element LUC
   

   Use

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-NBRE3

   Plasmid

   #208254

   Purpose

   pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells

   Depositor

   Douglas Kojetin

   Article

   Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

   Insert

   3X NBRE-LUC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cag.Luc.puro

    Plasmid

    #74409

    Purpose

    Constitutive Luciferase expression in mammalian cells along with puromycin selection

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR223-BALF4

    Plasmid

    #195835

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF4 (BALF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAVS1-phiC31-LP-TC

    Plasmid

    #183755

    Purpose

    AAVS1 targeting construct with the phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR-HOT-Clover-BlastR

    Plasmid

    #138569

    Purpose

    Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    Clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Use

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGART-GFP

    Plasmid

    #99106

    Purpose

    Expresses human GART-GFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    GART (GART Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A65T, L68V, and A72S mutations in GFP gene; kanR/…

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LAMP1-SBP-GFP

    Plasmid

    #120172

    Purpose

    Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Lysosomal-associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-opto-hCaspase-8

    Plasmid

    #208786

    Purpose

    Inducibly expresses human Caspase 8 fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase 8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-ATN1

    Plasmid

    #16179

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Atrophin 1 (ATN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-EF1a-FLEX-GT

    Plasmid

    #26198

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    TVA mammalian codon optimized

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFPN1-ATG9A

    Plasmid

    #198529

    Purpose

    Expression of ATG9A-GFP fusion protein in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations in codons 4 and 775

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA-h-mmPtch1-B-HA-TagBFP

    Plasmid

    #120910

    Purpose

    Expresses BFP tagged Ptch1-B

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-opto-hCaspase-4 dCARD

    Plasmid

    #208785

    Purpose

    Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry Caspase4 dCARD (CASP4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-GFP-FUS-IDR

    Plasmid

    #183236

    Purpose

    Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Use

    Tags

    FLAG tag and Fusion to an IDR from the FUS protei…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLPC-FLAG.Mm.EPC2

    Plasmid

    #184649

    Purpose

    Retroviral expression of mouse EPC2

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc2 (Epc2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLPC-FLAG.Mm.EPC1

    Plasmid

    #184648

    Purpose

    Retroviral expression of mouse EPC1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    EPC1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSC5

    Plasmid

    #188602

    Purpose

    Superior Conjugative Plasmid to Diverse Yeast

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1

    Plasmid

    #233078

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTRIP-CMV-tagRFP-FLAG-cGAS

    Plasmid

    #86676

    Purpose

    Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    tagRFP-FLAG-cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSEP-LAMP1-RFP

    Plasmid

    #167970

    Purpose

    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

    Depositor

    Juan Bonifacino

    Article

    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    SEP and mRFP

    Expression

    Mammalian

    Mutation

    D50E

    Promoter

    CMV

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Hsp68-LacZ-Gateway

    Plasmid

    #37843

    Depositor

    Nadav Ahituv

    Article

    Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Mouse Hsp68 minimal promoter

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Str-Ii_SBP-EGFP-Golgin84

    Plasmid

    #65303

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTRIP-SFFV-EGFP-NLS

    Plasmid

    #86677

    Purpose

    Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    EGFP-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR223-EBNA1

    Plasmid

    #195827

    Purpose

    Gateway Entry Clone of Epstein-Barr virus EBNA1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA1 (EBNA-1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-N1_MeCP2(WT)

    Plasmid

    #110186

    Purpose

    Expression of mouse MeCP2 (WT) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EGFP gRNA (BRDN0000561167)

    Plasmid

    #80034

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR-eGFP-attP(bxb)-*BsdR

    Plasmid

    #183751

    Purpose

    Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBR_attB(bxb)_ccdB_lox

    Plasmid

    #183763

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ccdB
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge