167,433 results

1. pET28.His.3C.Tau2N4R

   Plasmid

   #226396

   Purpose

   Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau2N4R (MAPT Human, synthetic)
   

   Tags

   His.3C

   Expression

   Bacterial

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CapChR2_pmScarlet-N1

   Plasmid

   #188032

   Purpose

   Expresses CapChR2-mScarlet in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. nuclear NAD+ biosensor

   Plasmid

   #186789

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbS5c-mCherry-AsLOV2*(543)

   Plasmid

   #202712

   Purpose

   Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Insert

   mCherry-AsLOV2*(543)
   

   Expression

   Bacterial

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available Since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-hChR2(H134R)-EYFP (AAV Retrograde)

   Viral Prep

   #26973-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   EYFP

   Available Since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCS mCherry

   Plasmid

   #91769

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Cherry
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   T7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPSU10-30-50-100

    Plasmid

    #173838

    Purpose

    Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF155 pcDNA3.1(+)SOD1G93A

    Plasmid

    #26401

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Mammalian

    Mutation

    G93A

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGD-p19

    Plasmid

    #196326

    Purpose

    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

    Depositor

    Zhenghe Li

    Article

    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

    Insert

    tomato bushy stunt virus p19
    

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral Prep

    #100850-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (#100850). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CaMKIIa-EGFP (AAV5)

    Viral Prep

    #50469-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    CaMKII

    Tags

    EGFP

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pFYF1320 EGFP Site#1

    Plasmid

    #47511

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB-gDA3m

    Plasmid

    #208698

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Has Service

    AAV1

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. rtTA-N144

    Plasmid

    #66810

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    Reverse tetracycline-controlled transactivator
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. dTomato-2xrGBD

    Plasmid

    #129625

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    dTomato-2xrGBD
    

    Expression

    Mammalian

    Promoter

    CMVdel

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-FLAG-hSF3B1-K700E

    Plasmid

    #82577

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-K700E (SF3B1 Human)
    

    Tags

    FLAG peptide

    Expression

    Mammalian

    Mutation

    K700E

    Promoter

    CMV

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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