We narrowed to 11,219 results for: NSI

1. pBad-CA TAG126

   Plasmid

   #105836

   Purpose

   Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 126

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGB Tnos:NptII:Pnos (GB0226)

   Plasmid

   #68212

   Purpose

   Transcriptional unit for kanamycin resistance gene plant expression

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   NptII
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-CRY2-mCherry

   Plasmid

   #178525

   Purpose

   Expresses CRY2-mCherry in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CRY2-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-blueCKAR(T/A)

   Plasmid

   #118473

   Purpose

   Negative-control mutant for blueCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueCKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHsp18.2 (GB0153)

   Plasmid

   #68171

   Purpose

   Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtHsp18.2 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BAS288 C terminal mKO2 natR

   Plasmid

   #74083

   Purpose

   S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   mKO2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. NELFE-mOrange pHis-//

   Plasmid

   #53303

   Purpose

   Expresses His-tagged NELF-E-mOrange fusion protein.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   NELF-E protein (Nelf-E Fly)
   

   Use

   Tags

   His and mOrange

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMM2-rPMCA2a

   Plasmid

   #47762

   Purpose

   mammalian expression of rat PMCA2a

   Depositor

   John Penniston, Emanuel Strehler

   Article

   Elwess et al J Biol Chem. 1997 Jul 18;272(29):17981-6

   Insert

   PMCA2a (Atp2b2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pHH0103_VAV2-2/2

   Plasmid

   #91506

   Purpose

   Protein expression and purification of human SH3 domain construct VAV2-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   VAV2-2/2 (VAV2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHsp70B (GB0155)

    Plasmid

    #68172

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp70B promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

    Plasmid

    #112496

    Purpose

    Expresses channel rhodopsin in astrocytes

    Depositor

    Baljit Khakh

    Article

    Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

    Insert

    ChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    H134R

    Promoter

    GfaABC1D

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PXPR007 sgGFP-2

    Plasmid

    #74961

    Purpose

    Cas9 + sgGFP-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_VAV3-1/2

    Plasmid

    #91528

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-1/2 (VAV3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHH0103_SORBS3-1/3

    Plasmid

    #91534

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS3-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS3-1/3 (SORBS3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_VAV3-2/2

    Plasmid

    #91524

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-2/2 (VAV3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_SH3YL1-1/1

    Plasmid

    #91485

    Purpose

    Protein expression and purification of human SH3 domain construct SH3YL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3YL1-1/1 (SH3YL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_NEBL-1/1

    Plasmid

    #91383

    Purpose

    Protein expression and purification of human SH3 domain construct NEBL-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NEBL-1/1 (NEBL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_NPHP1-1/1

    Plasmid

    #91358

    Purpose

    Protein expression and purification of human SH3 domain construct NPHP1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NPHP1-1/1 (NPHP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_PACSIN1-1/1

    Plasmid

    #91372

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN1-1/1 (PACSIN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_SH3D21-1/3

    Plasmid

    #91296

    Purpose

    Protein expression and purification of human SH3 domain construct SH3D21-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3D21-1/3 (SH3D21 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHH0103_MYO15A-2/2

    Plasmid

    #91291

    Purpose

    Protein expression and purification of human SH3 domain construct MYO15A-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MYO15A-2/2 (MYO15A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS-I-Sce1-slc6a3[11kb]:egfp

    Plasmid

    #53240

    Purpose

    allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

    Depositor

    Edward Burton

    Article

    Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

    Insert

    DAT-GFP
    

    Use

    Cloning

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDelila (GB0079)

    Plasmid

    #68200

    Purpose

    Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Delila coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRosea (GB0026)

    Plasmid

    #68194

    Purpose

    Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    A. majus Rosea coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBad-CA TAG20

    Plasmid

    #105666

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 20

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-nef-CIAO-TVA-mCherry

    Plasmid

    #149295

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Start codon removed.

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MS2-adRNA (5'UAG)

    Plasmid

    #170126

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'UAG)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BoxB-MS2 adRNA (RAB7A-UAG site)

    Plasmid

    #170138

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(UAG)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEmerald-PDHA1-N-10

    Plasmid

    #54225

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

31. pRenilla (GB0056)

    Plasmid

    #68198

    Purpose

    Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BAS292 C terminal SF-GFP kanR

    Plasmid

    #74087

    Purpose

    S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    SF-GFP recoded for S.pombe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pHH0103_PPP1R13L-1/1

    Plasmid

    #91509

    Purpose

    Protein expression and purification of human SH3 domain construct PPP1R13L-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PPP1R13L-1/1 (PPP1R13L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+) neo with Smad5 CDS

    Plasmid

    #61800

    Purpose

    pcDNA3.1(+) with full length mouse Smad5 CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Smad5 CDS (Smad5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pExT

    Plasmid

    #36889

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO.1-Teton-puro-shFAK #1

    Plasmid

    #198757

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #1 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLKO.1-Teton-puro-shFAK #2

    Plasmid

    #198758

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #2 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pExG

    Plasmid

    #36888

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PSUC2-yEGFPCLN2PEST-pRS406

    Plasmid

    #64405

    Purpose

    SUC2 promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    SUC2 promoter (SUC2 Budding Yeast)
    

    Use

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Mutation

    Promoter

    SUC2

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. BAS287 C terminal E2C hygBR

    Plasmid

    #74082

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only