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Showing: 2701 - 2720 of 19231 results

  5. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200402
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200402-plasmid-map-sequence-id-396788

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  7. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Bing Yang
    Article
    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Available Since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213049-plasmid-map-sequence-id-422145

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  8. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available Since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  16. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

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Showing: 2701 - 2720 of 19231 results