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We narrowed to 11,816 results for: nts

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Showing: 2701 - 2720 of 11816 results

  2. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

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  3. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral Prep
    #104496-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (#104496). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE plasmid DNA. CAG-driven, Cre-dependent jGCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    CAG
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pBSFI-myr-akt-S473A

    Plasmid
    #49190
    Purpose
    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector
    Depositor
    Peter Vogt
    Article
    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Adaptor plasmid
    Tags
    HA tag and myristolation signal
    Mutation
    S473A
    Promoter
    T7
    Available Since
    Dec. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. AD59_pEP.DonorCLYBL.TS

    Plasmid
    #199227
    Purpose
    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199227-plasmid-map-sequence-id-391392

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Showing: 2701 - 2720 of 11816 results