We narrowed to 17,803 results for: ERG

1. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

   Plasmid

   #154826

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   mCherry
   NeoR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL Blast Twist ER

   Plasmid

   #18799

   Depositor

   Bob Weinberg

   Article

   Mani et al Cell. 2008 May 16. 133(4):704-15.

   Insert

   twist (Twist1 Mouse)
   

   Use

   Retroviral

   Tags

   ER hormone binding domain

   Expression

   Mammalian

   Mutation

   G525R mutation in the ER. The presence of the G52…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA

   Plasmid

   #108730

   Purpose

   Expresses aa271 to C-terminus of Cre recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa271 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

   Plasmid

   #127355

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA

   Plasmid

   #108747

   Purpose

   Expresses N-terminus to aa27 of Flp recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment N-terminus to aa27
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

   Plasmid

   #127356

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-2T-GST-eIF4E K119A

   Plasmid

   #112818

   Purpose

   Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Eif4e (Eif4e Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K119 mutated to A, increasing affinity for cap st…

   Promoter

   T7

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUG-natNT2

   Plasmid

   #110922

   Purpose

   expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   nat
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   AgTEF1

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA

   Plasmid

   #108748

   Purpose

   Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa28 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

    Plasmid

    #31483

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Traffic Light 2.0 series have a +3 PEST sequence …

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCVL.SSA TLR (Sce)

    Plasmid

    #45576

    Purpose

    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. HSV-DYN-hM4Di

    Plasmid

    #53327

    Purpose

    Dynorphin promoter driving hM4Di

    Depositor

    John Neumaier

    Article

    Ferguson et al Nat Neurosci. 2011 Jan;14(1):22-4. doi:

    Insert

    hM4Di (Pdyn Rat)
    

    Use

    Hsv

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Rat dynorphin promoter ( -1858 to +135)

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

    Plasmid

    #186993

    Purpose

    AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-s1006-gggs-d122, non-targeting crRNA guide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    s1006-gggs-d1221 deletion

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-lincRNA-RoR

    Plasmid

    #45763

    Depositor

    George Daley

    Article

    Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

    Insert

    lincRNA-RoR cDNA (LINC-ROR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

    Plasmid

    #186987

    Purpose

    AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-s1006-gggs-d122, Gluc crRNA guide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    s1006-gggs-d1221 deletion

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLNCX2-NGN2-IRES-GFP

    Plasmid

    #90212

    Purpose

    To convert human fetal lung fibroblasts into induced cholinergic neurons (hiCN) in combination with two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

    Insert

    NGN2-IRES-GFP (NEUROG2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

    Plasmid

    #172514

    Purpose

    Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. scAAV-Mlc2v-MPRA

    Plasmid

    #182649

    Purpose

    Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    rat Mlc2v

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Use

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pHW581

    Plasmid

    #198818

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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