We narrowed to 4,418 results for: BRE

1. pLKO.1-shLuc.mKO2

   Plasmid

   #85224

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SYKA-c020

   Plasmid

   #175489

   Purpose

   Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

   Depositor

   Opher Gileadi

   Article

   Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

   Insert

   SYK (SYK Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HiBiT-FZD6

   Plasmid

   #195844

   Purpose

   Expresses human FZD6 with an N-terminal HiBiT; contains M345L

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD6 (FZD6 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   M345L

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PiggyBac-FLAG-mProser1-IRES-Blast

   Plasmid

   #226172

   Purpose

   Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

   Depositor

   Kasper Rasmussen

   Article

   Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

   Insert

   Proline Serine Rich protein 1 (Proser1 Mouse)
   

   Use

   Tags

   2X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2K-LRL-IRES-Luc2

   Plasmid

   #109230

   Purpose

   For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

   Insert

   LRL and Luc2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe-Puro-IKBalpha-wt

   Plasmid

   #15290

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pLV-CMV-XRCC1-EGFP-Hygro

   Plasmid

   #176062

   Purpose

   EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   X-ray repair cross complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-OSF-PP-human-CHMP3

   Plasmid

   #154180

   Purpose

   expresses human CHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3 (CHMP3 Human)
   

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-rBrokenheart

    Plasmid

    #203394

    Purpose

    AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    Brokenheart
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-Rab8a-DN (T22N)

    Plasmid

    #101049

    Purpose

    Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    CMV

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMVA2

    Plasmid

    #119951

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe RFP1-KRT5 hygro

    Plasmid

    #58493

    Purpose

    mammalian expression of mRFP1-tagged human KRT5

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    KRT5 (KRT5 Human)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. px330 Rosa26 AscI LH291Gu1 (LH500)

    Plasmid

    #99628

    Purpose

    Plasmid used to target the pre-modified Rosa26 allele with Cas9

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid

    #109428

    Purpose

    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry T2A GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Inserted 43 base pairs into mCherry to create rep…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac1_GI.1_N116C-G193C

    Plasmid

    #162580

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Asparagine 116 was mutated to Cysteine and Glycin…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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