We narrowed to 9,855 results for: control

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DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucNanobody IRES EGFP

Plasmid

#136619

Purpose

Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-EnhancerNb-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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UASGAL-Pgal

Plasmid

#60338

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Mutation

OsTIR1 codon optimized for human cells

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ChR2-mRuby2-ST

Plasmid

#109125

Purpose

Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChR2-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET41c-PhTET2

Plasmid

#182428

Purpose

Expresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli

Depositor

Bruno Franzetti

Article

Gauto et al Nat Commun. 2022 Apr 8;13(1):1927. doi:

Insert

Tetrahedral aminopeptidase TET2 from P. hirokoshii

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Cdk4-DN-HA

Plasmid

#1877

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2 PCNA GFP

Plasmid

#105937

Purpose

in vitro transcription, cell cycle nuclear marker

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

PCNA (PCNA Human)

Tags

GFP

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh20.1 GFP

Plasmid

#209106

Purpose

GFP expressing scramble of shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh20.1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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Cdk6-DN-HA

Plasmid

#1869

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Mito-CaNAR2

Plasmid

#64731

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Tags

Cerulean3, N-terminal 30 amino acids from DAKAP1,…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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(SUMO)5

Plasmid

#126949

Purpose

Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-G97 IRES EGFP

Plasmid

#136620

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)1

Plasmid

#127089

Purpose

Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP human SUMO3 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMZ-178

Plasmid

#195426

Purpose

Transient, constitutive expression of synZiFTR: ZF3-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-185

Plasmid

#195433

Purpose

Transient, constitutive expression of synZiFTR: ZF10-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXEE401BG

Plasmid

#91717

Purpose

CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-hZO-1-HA

Plasmid

#236528

Purpose

For expression of ZO-1 (human) in mammalian cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Tags

HA and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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