We narrowed to 4,533 results for: Gaa

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pSMP-EZH2_3

Plasmid

#36389

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-PH-SWAP70

Plasmid

#84582

Purpose

Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 (SWAP70 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSMP-Bmi1_3

Plasmid

#36354

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSCL.177

Plasmid

#184998

Purpose

Express -Eco1 EMX1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

EMX1 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p CIneo-RL-Let7-3xBulgeB-mut

Plasmid

#115369

Purpose

Expression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

Plasmid

#191397

Purpose

sgRNA targeting alpha-satellites on human chromosome 9

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

sgRNA targeting alpha satellites on human chromosome 9

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB3053(gRNA X-2, XI-5, XII-4)

Plasmid

#73295

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Arhgap20-guides

Plasmid

#254763

Purpose

Arhgap20 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Arhgap20 (arhgap20 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pGGA-FT guide RNA1_7

Plasmid

#246797

Purpose

A GreenGate entry vector containing a guide RNA expression cassette targeting the AtFT gene with 7 different gRNAs

Depositor

Danhua Jiang

Insert

AtU6-1 promoter-FT guide RNA1-AtU6-1 promoter-FT guide RNA3- (FT Synthetic, Mustard Weed)

Use

CRISPR; Greengate cloning entry vector

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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hSTX4(1-271,272C)

Plasmid

#206031

Purpose

Bacterial expression of human Syntaxin 4 without transmembrane helix and a C-terminal cysteine

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Syntaxin-4 (STX4 Human)

Tags

his-tag (removable with thrombin)

Expression

Bacterial

Mutation

deleted amino acids 272-297 (encoding the transme…

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW306_U6-sgRNA-EMX1

Plasmid

#244824

Purpose

Mammalian expression of SpyCas9 single guide RNA targeting EMX1

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-W308_guide-CBh-hSpCas9

Plasmid

#188546

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Promoter

U6

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSC51

Plasmid

#104825

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.12g075700, Glyma.11g145900

Use

CRISPR

Expression

Plant

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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CFDP1 C12.4 gRNA

Plasmid

#90625

Purpose

3rd generation lentiviral gRNA plasmid targeting human CFDP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CFDP1 (Guide Designation C12.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-IL6

Plasmid

#111933

Purpose

Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Interleukin 6 (IL6 Human)

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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VAMP3-mCherry

Plasmid

#92423

Purpose

Expression of VAMP3 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAP05

Plasmid

#186261

Purpose

sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

sfGFP
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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VAMP8-mCherry

Plasmid

#92424

Purpose

Expression of VAMP8 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP8 (Vamp8 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

A36S (Please see Depositor Comments below)

Promoter

CMV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLVUTHshGATA1-tTR-KRAB

Plasmid

#11650

Purpose

Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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WDFY2-GFP

Plasmid

#193636

Purpose

Expresses GFP-tagged WDFY2 in mammalian cells

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

WDFY2 (Vamp3 Human)

Tags

GFP

Expression

Mammalian

Mutation

GFP

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-irf3-shrna5

Plasmid

#127649

Purpose

Knock-down of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 shRNA (IRF3 Human)

Use

Lentiviral

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pT2/sh-Col1a1/GFP4_Seq1.2

Plasmid

#201403

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shJUND puro

Plasmid

#58698

Purpose

Lentiviral shRNA vector for knockdown of human JUND

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shJUND (JUND Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pMKO.1-shBirc5

Plasmid

#160949

Purpose

Birc5 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Birc5 shRNA (Birc5 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-PH-SWAP70(R223E, R224E)

Plasmid

#84583

Purpose

Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306, mut…

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
shMYB-2

Plasmid

#247031

Purpose

Used for a knockdown of MYB in human Megakaryocyte/Erythroid Progenitors

Depositor

Diane Krause

Article

Sanada et al Blood. 2016 Aug 18;128(7):923-33. doi: 1

Insert

shMYB-2 (MYB Human)

Use

Lentiviral and RNAi

Promoter

U6

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP3(S48A)-mCherry

Plasmid

#193635

Purpose

Expresses S48A phosphodead VAMP3 fused to mCherry

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

changed serine 48 to alanine

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP3(S48E)-mCherry

Plasmid

#193637

Purpose

Expresses S48E phosphomimetic VAMP3 fused to mCherry

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

changed serine 48 to alanine

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) WT

Plasmid

#206028

Purpose

Expresses the SNARE motif of wildtype human VAMP3 in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) S44A

Plasmid

#206029

Purpose

Expresses the SNARE motif of human VAMP3 mutant S44A in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Mutation

changes serine 44 to alanine

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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