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We narrowed to 12,317 results for: LEA

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Showing: 261 - 280 of 12317 results

  2. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45945-plasmid-map-sequence-id-116251

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  5. pX330-Cetn1/1

    Plasmid
    #50718
    Purpose
    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Publication
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50718-plasmid-map-sequence-id-75953

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  9. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65767-plasmid-map-sequence-id-471631

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  15. pTP1005

    Plasmid
    #104160
    Purpose
    Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)
    Depositor
    Dirk Görlich
    Publication
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104160-plasmid-map-sequence-id-200057

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Showing: 261 - 280 of 12317 results